Difference of SNRPN Methylation Status and Its mRNA Expression in Testes Between Cattle-Yaks and Their Parents

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dc.contributor.author Zeng-xiang, PAN en
dc.contributor.author Zhen-shan, LIU en
dc.contributor.author Yin-xia, LI en
dc.contributor.author Sha-li, YU en
dc.contributor.author Ming-gui, LI en
dc.contributor.author Zhuang, XIE en
dc.contributor.author Qi-fa, LI en
dc.date.accessioned 2012-06-26T22:40:58Z en
dc.date.issued 2010 en
dc.identifier.citation Scientia Agricultura Sinica 43:4709-4716 Article number 22 2010 en
dc.identifier.issn 0578-1752 en
dc.identifier.uri http://hdl.handle.net/2292/19181 en
dc.description.abstract 【Objective】 In order to reveal the apparent genetic mechanism of cattle-yaks about male sterile, DMR of SNRPN methylation status and its mRNA expression in testes between cattle-yaks and their parents were studied. 【Method】 The 5′-flanking sequence of SNRPN in yak was cloned and sequenced by the primers which designed on the basis of sequence of cattle SNRPN DNA. In cattle-yaks and their parents’ testes, the methylation status of SNRPN 5′-flanking was detected using sodium bisulfite sequencing and the SNRPN mRNA expression levels were also detected by Real-time PCR. 【Result】 The 5′-flanking sequence of SNRPN (1 137 bp) in yak was obtained, which the homology between yaks and cattle was 98.2%, and bioinformatics analysis suggested that the region contains the putative methylation-sensitive binding sites YY1 and SP1. The results showed that the methylation level of SNRPN gene in cattle-yaks (42.22%) was very significantly greater than that in cattle (21.08%) and yaks (20.81%) (P<0.01). The SNRPN expression level of cattle-yaks was lower than that of their parents in testes, but there was no significant difference (P>0.05). 【Conclusion】 The testicular methylation level of SNRPN gene in cattle-yaks was very significantly greater than that in cattle and yaks, the testicular SNRPN expression level of cattle-yaks was lower than that of their parents, indicating that the SNRPN gene maybe arrest meiotic process of spermatogenesis by its mRNA expression inhibition through the high methylation level of SNRPN gene DMR in cattle-yaks. en
dc.publisher China Academic Journals Electronic Publishing House en
dc.relation.ispartofseries Scientia Agricultura Sinica en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Difference of SNRPN Methylation Status and Its mRNA Expression in Testes Between Cattle-Yaks and Their Parents en
dc.type Journal Article en
dc.identifier.doi 10.3864/j.issn.0578-1752.2010.22.019 en
pubs.begin-page 4709 en
pubs.volume 43 en
dc.rights.holder Copyright: China Academic Journals Electronic Publishing House en
pubs.end-page 4716 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 343759 en
pubs.number 22 en
pubs.record-created-at-source-date 2012-04-18 en


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