Potential mechanism of augmentation of wound healing by anti-inflammatory and matrix metalloproteinase inhibitory effects of manuka and kanuka honey

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dc.contributor.advisor Ferguson, L en
dc.contributor.advisor Schlothauer, R en
dc.contributor.author Tomblin, Victoria en
dc.date.accessioned 2012-07-12T23:04:06Z en
dc.date.issued 2012 en
dc.identifier.uri http://hdl.handle.net/2292/19305 en
dc.description Full text is available to authenticated members of The University of Auckland only. en
dc.description.abstract The role of honey in wound healing, including as an antioxidant, an anti-inflammatory agent and inhibitor of the matrix metalloproteinase (MMP) family is gathering worldwide attention. This research examines some effects of honey on wound healing to ascertain its efficacy and potential as a treatment option. MMPs, highly expressed during episodes of injury and disease, play significant roles in physiological processes, such as proteolytic degradation and tissue remodelling. MMP-1, MMP-2 and MMP-9 perform crucial functions in wound healing, yet require tight control of their activation and expression to ensure normal wound repair. Where this control fails, chronic non-healing wounds develop. Four honeys were selected for this research, viz. manuka, kanuka (from a related floral source), a manuka/kanuka blend, and clover honey. Clover honey acted as a reference honey due to the absence of many therapeutic properties found in manuka and kanuka. Both extracted honey phenolics and crude honey extracts were used. MMP-1, MMP-2 and MMP-9 were selected for their importance in wound healing. Lung (H460), prostate (Du145) and breast (T47D) cancer cell lines were selected due to their expression of these MMPs. 50% cell growth inhibition concentrations were ascertained using IC50 assays that established the degree to which honey treatments reduce cell growth and proliferation. MMP inhibition assays were undertaken to examine the efficacy of honey phenolics in inhibiting MMP activity, and RT-PCR and ELISA analyses determined the effect that honey treatment had on MMP expression and protein concentrations. Comet and anti-inflammatory assays tested additional effects of honey and its constituents. Results indicated that honey significantly influences the activity of MMPs, and had a range of effects on their expression and protein concentrations. The phenolic compounds present in honey were observed to confer much of the benefits. The influence of honey treatment on antioxidant activity was significant yet variable, while kanuka honey produced a powerful anti-inflammatory effect. Honey used in wound treatment has been shown to have positive benefits, especially inhibiting MMP activity, and there is clear evidence to support its use as a topical application for external wounds. Nevertheless, honey acts in contradictory ways through different mechanisms and pathways, making it difficult to quantify its true value in wound repair. Indications are that honey’s phenolic compounds are responsible for its beneficial properties, although the specific compounds involved remain uncertain. Further research is required to substantiate its benefits. en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof Masters Thesis - University of Auckland en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights Restricted Item. Available to authenticated members of The University of Auckland. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/nz/ en
dc.title Potential mechanism of augmentation of wound healing by anti-inflammatory and matrix metalloproteinase inhibitory effects of manuka and kanuka honey en
dc.type Thesis en
thesis.degree.grantor The University of Auckland en
thesis.degree.level Masters en
dc.rights.holder Copyright: The author en
pubs.author-url http://hdl.handle.net/2292/19305 en
pubs.elements-id 358341 en
pubs.record-created-at-source-date 2012-07-13 en


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