Abstract:
Glutamate represents one of the most important chemical signalling compounds in the central nervous system but not much is known about it outside of the brain. Increasing research indicates that glutamate and its receptors contribute to the functioning of peripheral tissues, including megakaryocytes and platelets. The main objective of this study was to characterise NMDA receptor expression in human platelets using flow cytometry and electron microscopy. Flow cytometry confirmed previous data obtained in this laboratory that NMDA receptor subunits: GluN1, GluN2A and GluN2D are present in platelets. The surface expression of GluN1 was low in resting platelets but increased after platelet activation with ADP. Permeabilisation procedure allowed increased amounts of GluN1, GluN2A and GluN2D to be detected in platelets, suggesting significant intracellular storage pools of these proteins, as in neurons. High-resolution electron microscopy was used to investigate sub-cellular localisation of NMDA receptor subunits in platelets. GluN1, GluN2A and GluN2D were detected within alpha and dense granules, open canalicular systems, underneath and on the surface of plasma membranes, and on platelet projections. In resting platelets, majority of the subunits were found intracellularly, including in granules and channels; whereas in activated platelets GluN proteins were frequently seen on platelet surfaces and projections. Expression of GluN proteins at points of contact between platelets suggests that NMDA receptors may convey signals during platelet-platelet interactions. The second part of this thesis evaluated the presence of anti-platelet antibodies in stroke patients, and sought clinical correlations. Stroke patients were found to have higher incidence of platelet-reactive antibodies, compared with healthy blood donors. Clinically, stroke patients who had anti-platelet antibodies were more likely to have larger strokes with more severe neurological deficit. Intriguingly, patients with antibodies showed better recovery from their presenting deficit, suggesting that anti-platelet antibodies induce functional effects in vivo. In support of the in vivo antibody functionality, antibody-positive patients had higher platelet counts, suggesting an increased compensatory platelet production. Contrary to our original hypothesis, others in the laboratory found no evidence that stroke antibodies target GluN1. Further work is required to identify antibody targets and elucidate NMDA receptor function in human platelets.