dc.contributor.advisor |
McGhee, C |
en |
dc.contributor.advisor |
Kalloniatis, M |
en |
dc.contributor.advisor |
Polkinghorne, P |
en |
dc.contributor.advisor |
Acosta, M |
en |
dc.contributor.author |
Feitosa De Souza, C |
en |
dc.date.accessioned |
2012-08-13T20:21:46Z |
en |
dc.date.issued |
2012 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/19434 |
en |
dc.description.abstract |
Rhegmatogenous retinal detachment (RRD) is an important indication for ophthalmic surgery and a major cause of poor vision recovery post-reattachment operations. Photoreceptor death and anatomical remodelling of the deafferented inner retina have been documented in experimental primary RRD. However, little is known about noncomplicated human RRD. Therefore, it is important to identify specific patterns of cellular remodelling in the human retina after RRD and assess if these changes are associated with altered function of the retinal circuitry. Given that glutamate is the major neurotransmitter in the vertebrate retina we studied the glutamate pathway, the amino acid neurotransmitter levels and the expression of its functional ionotropic receptors. Functional characterization of these ionotropic glutamate receptors was achieved by using a cation channel permeating probe named agmatine (1-amino-4-guanidobutane; AGB). The functional profile map of ionotropic glutamate receptors in normal human mid-peripheral retina revealed increased AGB permeability in the detached outer retina. Increased functionality of KA receptors among ON bipolar cells suggested a corrupted circuitry. We also compared the anatomical profile of normal human retina with that of retinal samples from RRD employing a range of macromolecular markers. In the RRD samples, we observed ectopic synaptic protein expression, abnormal projection of photoreceptor processes towards the inner retina, neuronal migration, horizontal cell and ON bipolar cell extensions towards the nuclear layers. Some of these anatomical changes are novel features unreported in primary cases of RRD. Deafferentation in retinal detachment is associated with alteration of the glutamatergic pathway. In one radiation retinopathy sample, amino acids redistribution suggested a metabolic remodelling common to retinal degeneration conditions. Lastly, we compared the metabolic profile using creatine transporter (CRT) immunolabelling. CRT was expressed in areas of intense metabolic activity such as photoreceptors and in selected cells inner retina cells. The absence of CRT to Müller cells harmonizes with the notion that glial cells are biosynthetical source of creatine, although its provision to other cells may not occur. The increased CRT immunolabelling localized to the outer retina in RRD may indicate a regional metabolic remodelling in photoreceptors. |
en |
dc.publisher |
ResearchSpace@Auckland |
en |
dc.relation.ispartof |
PhD Thesis - University of Auckland |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
http://creativecommons.org/licenses/by-nc-sa/3.0/nz/ |
en |
dc.title |
Anatomical and Functional Characterisation of the Normal and Detached Human Retina |
en |
dc.type |
Thesis |
en |
thesis.degree.grantor |
The University of Auckland |
en |
thesis.degree.level |
Doctoral |
en |
thesis.degree.name |
PhD |
en |
dc.rights.holder |
Copyright: The author |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.elements-id |
360149 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Optometry and Vision Science |
en |
pubs.record-created-at-source-date |
2012-08-14 |
en |
dc.identifier.wikidata |
Q112889638 |
|