Survival of green-lipped mussels (Perna canaliculus) in air: characterisation of glycolytic enzyme activity, HSP70 and metabolomic profiling

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dc.contributor.advisor Dunphy, B en
dc.contributor.advisor Ragg, N en Tagaloa, Angela en 2012-09-17T03:42:50Z en 2012 en
dc.identifier.uri en
dc.description Full text is available to authenticated members of The University of Auckland only. en
dc.description.abstract The Greenshell™ mussel, Perna canaliculus is New Zealand’s largest aquaculture export, earning more than USD200 million in 2010. The aquaculture sector has grown rapidly through the value-adding of product, with efforts to expand this industry further through live export to high demand markets in Europe, Asia and America. There are however constraints to achieving this, as extensive research is required to understand the biology of this species and how it tolerates aerial survival, as they can be subjected to a combination environmental stress. The research presented in this thesis was based on identifying good, average and poor aerial exposure survivor families, using 19 separate family lines from the Cawthron Institute in Nelson, and 4 families from SpatNZ. Representatives from these categories were further investigated to see if there were any biochemical parameters associated with these traits through the characterisation of anaerobic glycolytic enzymes, quantification of HSP70 production and creating a metabolite profile and determining of distinct biomarkers that may be used for future selective breeding programmes. There were significant differences in aerial survival capability between families, with first mortalities occurring at 3 days of emersion and total population mortality by 14 days. Shell length and wet weight were both found to be negatively correlated with survival. The posterior adductor muscle of six families from different temporal scales (Day=0, 4, 8) were used for opine dehydrogenase assays. The specific activity (i.μ) of both SDH and AlaDH were found at significant levels in all tissues with good and poor survivors reducing opine dehydrogenase activity by day 8, however average survivors maintained elevated levels of activity. ii For HSP70 production and metabolomics testing, the gill tissue of three family lines were tested along the same temporal scales as enzymes with inconclusive evidence of HSP70 production as recorded proportion of total protein in the tissue was at detected at low levels, however patterns in production were shown to correlate with aerial survival capability. The metabolite profile produced showed strong evidence for opine dehydrogenase activity and other related anaerobic glycolytic pathways in the posterior adductor muscle with metabolite concentration in families correlating with enzymatic activity and survival capability. The results from this thesis suggest that there is possibility in the use of the amino acids alanine and glycine, as well as the glycolytic intermediates succinic acid and fumaric as biomarkers of superior aerial survivors in P. canaliculus due to their higher activity in good aerial survivors at increasing days of aerial exposure. en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof Masters Thesis - University of Auckland en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights Restricted Item. Available to authenticated members of The University of Auckland. en
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dc.title Survival of green-lipped mussels (Perna canaliculus) in air: characterisation of glycolytic enzyme activity, HSP70 and metabolomic profiling en
dc.type Thesis en The University of Auckland en Masters en
dc.rights.holder Copyright: The author en
pubs.elements-id 361139 en
pubs.record-created-at-source-date 2012-09-17 en

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