Abstract:
The Aim of this research was to develop drug delivery system for Oxaliplatin, an antineoplastic agent used in treatment of cancer. In order to improve its pharmacokinetics and anticancer activity, Solid Lipid Nanoparitcle (SLN) was chosen as carrier system. Throughout the experiment Ibuprofen was chosen as the model drug. Many different parameters including lipid material, surfactant, organic solvents, and drug concentrations were experimented to obtain the optimum formulation. The drug containing SLNs were prepared with emulsion formation/Solvent evaporation method employed with ultrasonication. The size of SLNs ranged from 318.8nm to 1945.3nm; zeta potentials ranged from -2.68 to -21.97 mV; drug loading ranged from 21.44% to 38.96%. The SLN regarded as the best from 20 different formulations was prepared with 100 mg of compritol, dichloromethane, 5% of Tween 80, 10mg of drug. This SLN had an average particle size of 318.8 nm, polydipesrsity index of 0.314, zeta-potential of -15.9mV, and encapsulation of 30.2%. Oxaliplatin was loaded with the chosen formulation and the cytotoxicity test was conducted in comparison with commercial solution and empty SLN. Oxaliplatin nanoparticles exhibited the characteristics of SLN providing protection of drug and sustaining the release. Also empty nanoparticles proved SLN prepared from the developed method had no or very little inherent toxicity.