dc.contributor.advisor |
Mansell, C |
en |
dc.contributor.advisor |
Dunbar, R |
en |
dc.contributor.author |
Stirrat, Rewi |
en |
dc.date.accessioned |
2013-02-28T23:03:03Z |
en |
dc.date.issued |
2013 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/20093 |
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dc.description |
Full text is available to authenticated members of The University of Auckland only. |
en |
dc.description.abstract |
Lymph nodes (LNs) are secondary lymphoid organs in which lymphocytes encounter their cognate antigen and interact with stromal and myeloid tissue to launch an immune response. Antigen presenting cells (APCs) are critical to this process as their roles include the capture and presentation of antigen via type I and II Major Histocompatibility Complex (MHC) and regulation of lymphocyte activation and response. However, APC subsets in human lymph nodes are currently only partially defined by cellular marker co-localisation. APC subsets in diseased lymph nodes are even less well defined. In the case of Hodgkin Lymphoma (HL), APC markers such as CD68 have been associated with poor disease prognosis, yet the subsets of APCs expressing CD68 in both the cancerous and normal human lymph nodes have not been fully described. Intriguingly, APCs have also been described as polarising towards either tumour-promoting or tumour-suppressing roles in cancer. Signalling protein pSTAT6 was utilised as a marker for APC M2 polarisation. This thesis sought to better define the subsets of APCs in normal and HL LNs. Immunohistochemistry using multiple fluorescent markers was performed on normal and HL lymph nodes. Subsets of APCs in normal lymph nodes expressing CD68 were defined by marker repertoire and localisation; Tingible Body Macrophages (TBMs) (CD68+HAM56+CD163dimCD209-) were found within follicles, TBM-like APCs (CD68+HAM56+CD163+CD209-) were found throughout the paracortical and interfollicular regions, and Sinus Type I (CD68-HAM56-CD209+CD163-) and Sinus Type II (CD68+HAM56-CD209+CD163+) APCs appeared throughout the lymphatic sinuses. APCs were observed in HL lymph nodes, but marker co-localisation was inconsistent with the subsets defined in normal lymph nodes. However, pSTAT6 presence was not associated with APCs, but was instead observed in follicular dendritic cells (FDCs) within some follicles of normal LNs and in the microenvironment of some HL LNs. pSTAT6 presence on FDCs was partially associated with proliferation marker Ki67 expression in surrounding lymphocytes. These observations raise questions about the validity of pSTAT6 as an indicator of M2 polarised APCs in HL, the role of FDCs in normal lymph node function, and whether pSTAT6 can help to characterise follicular activation markers in the normal and disease human LNs. |
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dc.publisher |
ResearchSpace@Auckland |
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dc.relation.ispartof |
Masters Thesis - University of Auckland |
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dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
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dc.rights |
Restricted Item. Available to authenticated members of The University of Auckland. |
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dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
http://creativecommons.org/licenses/by-nc-sa/3.0/nz/ |
en |
dc.title |
Antigen-Presenting Cell Subset Classification and Functional Marker Observations in Normal and Hodgkin Lymphoma Human Lymph Nodes |
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dc.type |
Thesis |
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thesis.degree.grantor |
The University of Auckland |
en |
thesis.degree.level |
Masters |
en |
dc.rights.holder |
Copyright: The Author |
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pubs.elements-id |
373766 |
en |
pubs.record-created-at-source-date |
2013-03-01 |
en |
dc.identifier.wikidata |
Q112901622 |
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