Essential replication functions of the F plasmid

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dc.contributor.advisor Prof. P. Bergquist and Dr D. Lane en Watson, Lynley A. en 2007-11-14T02:41:42Z en 2007-11-14T02:41:42Z en 1983 en
dc.identifier.citation Thesis (PhD--Cell Biology)--University of Auckland, 1983. en
dc.identifier.uri en
dc.description.abstract The work in this thesis concerns the analysis of essential replication genes of the F plasmid of Escherichia coli. The techniques principally employed were the cloning of restriction fragments, transposition mutagenesis, maxicell analysis of proteins, and DNA sequencing. This work led to the following findings. 1. A 1.15kb region of f5 was shown to contain all the functions required for initiation of F replication. Insertions of a transposon within this 1.15kb region abolished mini-F replication. 2. A mini-F plasmid was constructed which constitutes the smallest F derivative so far reported. This plasmid has an elevated copy number, as a result of deletion of the incC region. 3. At least two proteins specified by the essential F replication region were identified in maxicells. The promoter fragments of two mini-F genes were fused to a β-qalactosidase gene and demonstrated to have low but significant in vivo activities. en
dc.format Scanned from print thesis en
dc.language.iso en en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.relation.isreferencedby UoA219342 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. en
dc.rights.uri en
dc.title Essential replication functions of the F plasmid en
dc.type Thesis en Biology en The University of Auckland en Doctoral en PhD en
dc.subject.marsden Fields of Research::270000 Biological Sciences en
dc.rights.holder Copyright: The author en
pubs.local.anzsrc 0601 - Biochemistry and Cell Biology en Faculty of Science en

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