Sequence analysis and characterization of the hmw gene cluster of Mycoplasma pneumoniae

Show simple item record Dirksen, LB en Proft, Thomas en Hilbert, H en Plagens, H en Herrmann, R en Krause, DC en 2013-10-03T00:08:23Z en 1996 en
dc.identifier.citation Gene 171(1):19-25 24 May 1996 en
dc.identifier.issn 0378-1119 en
dc.identifier.uri en
dc.description.abstract Mycoplasma pneumoniae (Mp) cytadherence requires the proper anchoring of cytadhesin proteins in the mycoplasmal membrane at an attachment organelle through their interaction with a cytoskeleton-like network of accessory proteins that includes HMW1 and HMW3. Approximately 8.25 kb of Mp DNA was sequenced, beginning at the 3' end of the hmw3 gene and continuing through hmw1. Comparison of the resulting deduced amino acid (aa) sequence with N terminus and internal peptide aa sequences from purified HMW1 permitted definitive identification of hmw1. HMW1 was characterized with respect to structure, hydrophobicity, possible phosphoacceptor sites and expression of the Mp recombinant protein in Escherichia coli. In addition, HMW1 membrane topography was examined for antibody accessibility on the mycoplasmal surface. hmw3 and hmw1 flank four open reading frames (ORFs) spanning approximately 4.3 kb and in the same orientation as the hmw genes. The sequences of their deduced products were evaluated for likely structural features and comparison with protein data banks. Finally, the Mp rpsD analog was identified immediately downstream from hmw1. en
dc.relation.ispartofseries Gene en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.title Sequence analysis and characterization of the hmw gene cluster of Mycoplasma pneumoniae en
dc.type Journal Article en
dc.identifier.doi 10.1016/0378-1119(96)00050-9 en
pubs.issue 1 en
pubs.begin-page 19 en
pubs.volume 171 en
dc.identifier.pmid 8675025 en
pubs.end-page 25 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 38803 en Medical and Health Sciences en Medical Sciences en Molecular Medicine en Science en Science Research en Maurice Wilkins Centre (2010-2014) en
pubs.record-created-at-source-date 2010-09-01 en
pubs.dimensions-id 8675025 en

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