The development of rapid freezing techniques, and their application to an electron microscopical study of phloem structure

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dc.contributor.advisor Associate Professor S. Bullivant en
dc.contributor.advisor Dr R.L. Bieleski en
dc.contributor.author Dempsey, Graeme Peter en
dc.date.accessioned 2007-11-16T02:29:34Z en
dc.date.available 2007-11-16T02:29:34Z en
dc.date.issued 1975 en
dc.identifier.citation Thesis (PhD--Cell Biology)--University of Auckland, 1975. en
dc.identifier.uri http://hdl.handle.net/2292/2099 en
dc.description.abstract Preface This thesis is divided into two parts: Part I - The Use of Rapid Freezing Techniques to Study Unfixed, Non-Cryoprotected Animal and Plant Tissues by Freeze-Substitution and Freeze-Fracturing . Part II - The Distribution of P-Protein in Mature Sieve Elements of Phloem, and its Relation to Proposed Translocatory Mechanisms. The ultrastructure of the sieve elements of the phloem has remained a controversial issue for many years, mainly because of the extreme sensitivity of the cells to manipulation and conventional electron microscopical fixation methods. It was thought that rapid freezing, in association with freeze-substitution or freeze-fracturing, would stabilise the tissue and provide an image of the sieve element as little removed as possible from the in vivo condition. Thus, it was necessary to utilise a technique which froze tissue extremely rapidly, so that the use of cryoprotectants could be avoided. Therefore, the work reported in Part I of this thesis describes efforts to evaluate the available freezing techniques, and to adapt and test a suitable technique for the investigation of phloem ultrastructure. This technique, coupled with other conventional electron microscopical methods, is employed in Part II of this thesis to study the ultrastructure of mature sieve elements. en
dc.format Scanned from print thesis en
dc.language.iso en en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.relation.isreferencedby UoA217772 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title The development of rapid freezing techniques, and their application to an electron microscopical study of phloem structure en
dc.type Thesis en
thesis.degree.discipline Biological Sciences en
thesis.degree.grantor The University of Auckland en
thesis.degree.level Doctoral en
thesis.degree.name PhD en
dc.subject.marsden Fields of Research::270000 Biological Sciences::270100 Biochemistry and Cell Biology en
dc.rights.holder Copyright: The author en
pubs.local.anzsrc 0601 - Biochemistry and Cell Biology en
pubs.org-id Faculty of Science en


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