Microbial enzyme activity at the watershed scale: Response to chronic nitrogen deposition and acute phosphorus enrichment

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dc.contributor.author Mineau, MM en
dc.contributor.author Fatemi, FR en
dc.contributor.author Fernandez, IJ en
dc.contributor.author Simon, Kevin en
dc.date.accessioned 2014-02-03T01:25:25Z en
dc.date.issued 2014-01 en
dc.identifier.citation Biogeochemistry 117(1):131-142 Jan 2014 en
dc.identifier.issn 0168-2563 en
dc.identifier.uri http://hdl.handle.net/2292/21528 en
dc.description.abstract Microbial enzymes play a critical role in organic matter decomposition and enzyme activity can dynamically respond to shifts in inorganic nutrient and substrate availability, reflecting the nutrient and energy limitation of the microbial community. We characterized microbial enzyme response to shifting nitrogen (N) and phosphorus (P) availability across terrestrial and aquatic environments at the Bear Brook Watershed in Maine, the site of a whole-watershed N enrichment experiment. We compared activity of β-1,4-glucosidase (BG); β-1,4-N-acetylglucosaminidase (NAG); acid phosphatase (AP) in soil, leaf litter in terrestrial and stream habitats and stream biofilms in a reference and N enriched watershed, representing whole-ecosystem response to chronic N enrichment. In addition, we used shorter, experimental P enrichments to address potential P limitation under ambient and elevated N availability. We found that BG and NAG activity were not affected by the long-term N enrichment in either habitat. Enhanced P limitation due to N enrichment was evident only in the aquatic habitats with 5- and 8-fold higher treated watershed AP activity in stream biofilms and stream litter, respectively. Acute P additions reduced AP activity and increased BG activity and these effects were also most pronounced in the streams. The stoichiometry of enzyme activity was constrained across ecosystem compartments with regression slopes for lnBG:lnNAG, lnBG:lnAP, and lnNAG:lnAP close to 1, ranging 1.142-1.241. We found that microbial enzyme response to shifting N and P availability varied among watershed compartments, typically with stronger effects in aquatic habitats. This suggests that understanding the response of ecosystem function to disturbance at the watershed scale requires simultaneous consideration of all compartments. en
dc.publisher Springer Netherlands en
dc.relation.ispartofseries Biogeochemistry en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0168-2563/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Microbial enzyme activity at the watershed scale: Response to chronic nitrogen deposition and acute phosphorus enrichment en
dc.type Journal Article en
dc.identifier.doi 10.1007/s10533-013-9869-6 en
pubs.issue 1 en
pubs.begin-page 131 en
pubs.volume 117 en
pubs.end-page 142 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 380567 en
pubs.org-id Science en
pubs.org-id School of Environment en
dc.identifier.eissn 1573-515X en
pubs.record-created-at-source-date 2014-02-03 en

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