An integrated process for the recovery of clinically significant trace proteins from human plasma

Show simple item record

dc.contributor.advisor Professor A. Renwick en
dc.contributor.author Benny, Athol Graeme en
dc.date.accessioned 2007-12-02T22:36:12Z en
dc.date.available 2007-12-02T22:36:12Z en
dc.date.issued 1990 en
dc.identifier.citation Thesis (PhD--Biochemistry)--University of Auckland. en
dc.identifier.uri http://hdl.handle.net/2292/2157 en
dc.description.abstract Methods for the preparation of concentrates of factor VIII, factor IX, high purity factor IX, Cl esterase inhibitor, specific immunoglobulin and platelet factor XIII are described. These procedures were developed or modified with the aim of integration into an automated process that would allow sequential recovery of all the clinically significant trace proteins from a single plasma pool. Concomitant recovery of important proteins such as transferrin, alpha-1-antitrypsin and platelet-derived growth factor was considered. A high-purity factor VIII concentrate heat-treated at 80°C for 96 h was prepared by a process that incorporated heparin fractionation. This method was shown to be suitable for assimilation into an existing regional blood processing laboratory. Several ion-exchange procedures for the recovery of factor IX were evaluated and higher purification of a factor IX concentrate was achieved on a new cellulose-based chromatographic medium. A chromatographic procedure for the preparation of a heat-treated high-purity Cl esterase inhibitor concentrate was described and the performance of a new cellulose-based desalting medium was evaluated in comparison with ultrafiltration. A heat-treated specific immunoglobulin concentrate was prepared from side-stream fractions of an automated chromatographic process for the production of albumin concentrate, and a pilot study for the fractionation of outdated platelet concentrates was carried out with the aim preparing components of potential therapeutic value. See summary flow diagrams of fractionation processes included with references in the back of this thesis. en
dc.format Scanned from print thesis en
dc.language.iso en en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.relation.isreferencedby UoA405338 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title An integrated process for the recovery of clinically significant trace proteins from human plasma en
dc.type Thesis en
thesis.degree.discipline Biochemistry en
thesis.degree.grantor The University of Auckland en
thesis.degree.level Doctoral en
thesis.degree.name PhD en
dc.subject.marsden Fields of Research::270000 Biological Sciences::270100 Biochemistry and Cell Biology::270199 Biochemistry and cell biology not elsewhere classified en
dc.rights.holder Copyright: The author en
pubs.local.anzsrc 0601 - Biochemistry and Cell Biology en
pubs.org-id Faculty of Science en


Files in this item

Find Full text

This item appears in the following Collection(s)

Show simple item record

Share

Search ResearchSpace


Browse

Statistics