An integrated process for the recovery of clinically significant trace proteins from human plasma

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dc.contributor.advisor Professor A. Renwick en Benny, Athol Graeme en 2007-12-02T22:36:12Z en 2007-12-02T22:36:12Z en 1990 en
dc.identifier.citation Thesis (PhD--Biochemistry)--University of Auckland. en
dc.identifier.uri en
dc.description.abstract Methods for the preparation of concentrates of factor VIII, factor IX, high purity factor IX, Cl esterase inhibitor, specific immunoglobulin and platelet factor XIII are described. These procedures were developed or modified with the aim of integration into an automated process that would allow sequential recovery of all the clinically significant trace proteins from a single plasma pool. Concomitant recovery of important proteins such as transferrin, alpha-1-antitrypsin and platelet-derived growth factor was considered. A high-purity factor VIII concentrate heat-treated at 80°C for 96 h was prepared by a process that incorporated heparin fractionation. This method was shown to be suitable for assimilation into an existing regional blood processing laboratory. Several ion-exchange procedures for the recovery of factor IX were evaluated and higher purification of a factor IX concentrate was achieved on a new cellulose-based chromatographic medium. A chromatographic procedure for the preparation of a heat-treated high-purity Cl esterase inhibitor concentrate was described and the performance of a new cellulose-based desalting medium was evaluated in comparison with ultrafiltration. A heat-treated specific immunoglobulin concentrate was prepared from side-stream fractions of an automated chromatographic process for the production of albumin concentrate, and a pilot study for the fractionation of outdated platelet concentrates was carried out with the aim preparing components of potential therapeutic value. See summary flow diagrams of fractionation processes included with references in the back of this thesis. en
dc.format Scanned from print thesis en
dc.language.iso en en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.relation.isreferencedby UoA405338 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. en
dc.rights.uri en
dc.title An integrated process for the recovery of clinically significant trace proteins from human plasma en
dc.type Thesis en Biochemistry en The University of Auckland en Doctoral en PhD en
dc.subject.marsden Fields of Research::270000 Biological Sciences::270100 Biochemistry and Cell Biology::270199 Biochemistry and cell biology not elsewhere classified en
dc.rights.holder Copyright: The author en
pubs.local.anzsrc 0601 - Biochemistry and Cell Biology en Faculty of Science en

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