dc.contributor.author |
Chan, YK |
en |
dc.contributor.author |
Davis, P |
en |
dc.contributor.author |
Poppitt, Sally |
en |
dc.contributor.author |
Sun, Xueying |
en |
dc.contributor.author |
Greenhill, N |
en |
dc.contributor.author |
McGill, Anne-Thea |
en |
dc.contributor.author |
Krissansen, Geoffrey |
en |
dc.date.accessioned |
2012-03-18T18:40:53Z |
en |
dc.date.accessioned |
2014-08-04T07:15:21Z |
en |
dc.date.issued |
2012-04 |
en |
dc.identifier.citation |
Laboratory Animals, 2012, 46 (2), pp. 142 - 147 |
en |
dc.identifier.issn |
0023-6772 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/22644 |
en |
dc.description.abstract |
Blood is collected during animal experimentation to measure haematological and metabolic parameters. It cannot be assumed that circulating blood has the same composition irrespective of its location, and indeed, differences in the composition of blood sampled from the arterial and venous compartments have been reported. Here we investigated whether blood collected by cardiac puncture (CP) versus that collected following removal of the distal 1 mm of the tail tip (TT) differs with respect to glucose and lipid profiles in male C57BL/6J mice at 4, 7, 20 and 28 weeks of age. Blood was first collected from the TT of unanaesthetized mice, which were then immediately anaesthetized using ketamine/xylazine, and a second blood sample was collected by CP. The CP glucose concentration was significantly higher than TT glucose by a positive bias averaging +80% (P < 0.01), irrespective of the age of the mice. Conversely, the concentrations of the CP lipids, including total cholesterol, high-density lipoprotein cholesterol and triglyceride were lower than TT lipids by a negative bias averaging −25% (P < 0.05). These observations highlight the difficulty in measuring and comparing metabolic parameters such as glucose and lipid between one blood compartment and another. They illustrate the need to standardize sampling sites, especially when repeated blood sampling is required. |
en |
dc.publisher |
Royal Society of Medicine Press |
en |
dc.relation.ispartofseries |
Laboratory Animals |
en |
dc.relation.replaces |
http://hdl.handle.net/2292/14562 |
en |
dc.relation.replaces |
2292/14562 |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0023-6772/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Influence of tail versus cardiac sampling on blood glucose and lipid profiles in mice |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1258/la.2011.011136 |
en |
pubs.issue |
2 |
en |
pubs.begin-page |
142 |
en |
pubs.volume |
46 |
en |
dc.rights.holder |
Copyright:
Royal Society of Medicine Press |
en |
dc.identifier.pmid |
22398273 |
en |
pubs.end-page |
147 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
313140 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Biological Sciences |
en |
pubs.record-created-at-source-date |
2012-03-06 |
en |
pubs.dimensions-id |
22398273 |
en |