P2X Receptor Signaling Inhibits BDNF-Mediated Spiral Ganglion Neuron Development in the Neonatal Rat Cochlea

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dc.contributor.author Greenwood, Denise en
dc.contributor.author Jagger, Daniel en
dc.contributor.author Huang, Lin-Chien en
dc.contributor.author Hoya, Noriyuki en
dc.contributor.author Thorne, Peter en
dc.contributor.author Wildman, SS en
dc.contributor.author King, BF en
dc.contributor.author Pak, K en
dc.contributor.author Ryan, AF en
dc.contributor.author Housley, Gary en
dc.date.accessioned 2012-03-26T20:03:23Z en
dc.date.accessioned 2014-10-15T00:28:33Z en
dc.date.issued 2007 en
dc.identifier.citation Development, 2007, 134 pp. 1407 - 1417 (11) en
dc.identifier.issn 0950-1991 en
dc.identifier.uri http://hdl.handle.net/2292/23228 en
dc.description.abstract Type I and type II spiral ganglion neurons (SGN) innervate the inner and outer hair cells of the cochlea, respectively. This neural system is established by reorganization of promiscuous innervation of the hair cells, immediately before hearing is established. The mechanism for this synaptic reorganization is unresolved but probably includes regulation of trophic support between the hair cells and the neurons. We provide evidence that P2X receptors (ATP-gated ion channels) contribute such a mechanism in the neonatal rat cochlea. Single-cell quantitative RT-PCR identified the differential expression of two P2X receptor subunits, splice variant P2X2-3 and P2X3, in a 1:2 transcript ratio. Downregulation of this P2X2-3/3 receptor coincided with maturation of the SGN innervation of the hair cells. When the P2X2-3 and P2X3 subunits were co-expressed in Xenopus oocytes, the resultant P2X receptor properties corresponded to the SGN phenotype. This included enhanced sensitivity to ATP and extended agonist action. In P4 spiral ganglion explants, activation of the P2X receptor signaling pathway by ATP S or , MeATP inhibited BDNF-induced neurite outgrowth and branching. These findings indicate that P2X receptor signaling provides a mechanism for inhibiting neurotrophin support of SGN neurites when synaptic reorganization is occurring in the cochlea. en
dc.publisher Company of Biologists en
dc.relation.ispartofseries Development en
dc.relation.replaces http://hdl.handle.net/2292/15434 en
dc.relation.replaces 2292/15434 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from: http://www.sherpa.ac.uk/romeo/issn/0950-1991/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title P2X Receptor Signaling Inhibits BDNF-Mediated Spiral Ganglion Neuron Development in the Neonatal Rat Cochlea en
dc.type Journal Article en
dc.identifier.doi 10.1242/dev.002279 en
pubs.begin-page 1407 en
pubs.volume 134 en
dc.rights.holder Copyright: Company of Biologists en
dc.identifier.pmid 17329369 en
pubs.end-page 1417 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 75574 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Faculty Administration FMHS en
pubs.org-id FMHS Research Support en
pubs.org-id Medical Sciences en
pubs.org-id Physiology Division en
pubs.org-id Population Health en
pubs.org-id Audiology en
dc.identifier.eissn 1477-9129 en
pubs.record-created-at-source-date 2010-09-01 en
pubs.dimensions-id 17329369 en


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