Transcriptional promoters in a replication region of F plasmid

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dc.contributor.author Kennedy, Martin A. en
dc.date.accessioned 2008-04-09T01:28:47Z en
dc.date.available 2008-04-09T01:28:47Z en
dc.date.issued 1986 en
dc.identifier.citation Thesis (PhD--Cell Biology)--University of Auckland, 1986. en
dc.identifier.uri http://hdl.handle.net/2292/2482 en
dc.description.abstract This thesis describes aspects of genetic regulation within and near a replication origin (ori-1) of the F plasmid. A number of transcriptional promoters were isolated, precisely mapped, and characterized with respect to their strengths and modes of regulation. The principal techniques employed in these investigations were: "shotgun" molecular cloning of restriction fragments into a galactokinase-based promoter selection vector, assays for galactokinase activities, DNA sequencing and S1 nuclease mapping of transcripts. Major findings from this study can be summarized as follows: 1). Promoters for the essential replication genes pifC and E were cloned and shown to be autoregulated at the transcriptional level. 2). An E.coli protein, integration host factor (IHF), was found to modulate the activity of the pif operon promoter. 13). Two promoters which direct transcription in opposite directions from within the minimal ori-1 region were discovered. 4). Transcription from both ori-1 promoters was shown to be repressed by the mini-F encoded D protein. 5). Precise transcriptional startsites of the pifC gene and the two ori-1 promoters were determined. 6). A mini-F protein (D) was shown to resolve dimers of a plasmid which contains a site-specific recombination locus from near ori-1, and a facile assay system for this function was developed. en
dc.language.iso en en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.relation.isreferencedby UoA101298 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Transcriptional promoters in a replication region of F plasmid en
dc.type Thesis en
thesis.degree.discipline Cell Biology en
thesis.degree.grantor The University of Auckland en
thesis.degree.level Doctoral en
thesis.degree.name PhD en
dc.subject.marsden Fields of Research::270000 Biological Sciences::270100 Biochemistry and Cell Biology en
dc.rights.holder Copyright: The author en
pubs.local.anzsrc 0601 - Biochemistry and Cell Biology en
pubs.org-id Faculty of Science en


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