Calcium phosphates in Ca(2+)-fortified milk: phase identification and quantification by Raman spectroscopy

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dc.contributor.author Arifin, M en
dc.contributor.author Swedlund, Peter en
dc.contributor.author Hemar, Yacine en
dc.contributor.author McKinnon, IR en
dc.date.accessioned 2015-05-06T02:44:10Z en
dc.date.issued 2014-12 en
dc.identifier.citation Journal of Agricultural and Food Chemistry, 2014, 62 (50), pp. 12223 - 12228 en
dc.identifier.issn 1520-5118 en
dc.identifier.uri http://hdl.handle.net/2292/25419 en
dc.description.abstract Calcium phosphate nanoclusters (CPNs) are important for the structure, function, and nutrient density of many dairy products. Phosphorylated amino acids in caseins stabilize calcium phosphate as nanoclusters which are amorphous to X-ray diffraction and exist within casein micelles, and these CPNs play a key role in micelle stability. Addition of calcium to milk results in further calcium phosphate removal from the serum, and there is uncertainty about the nature of the material formed and its stability. In this work we investigate both the solution and colloidal phases in CaCl2 enriched bovine milk to identify, quantify, and determine the solubility of the calcium phosphate material formed in response to calcium addition to milk. The P-O stretching bands are quite distinct in the Raman spectra of the main synthetic calcium phosphate mineral phases, including the amorphous calcium phosphate phase. In response to adding between 5 and 40 mM CaCl2 to milk, the serum phosphate concentration decreased asymptotically from 7.5 ± 0.2 to 0.54 ± 0.05 mM. Using Raman spectroscopy with a combination of internal and external standards, it was possible to show that the calcium phosphate material formed after Ca(2+) addition to milk was the same as amorphous calcium phosphate nanoclusters present in the absence of added calcium. The use of an internal standard allowed a quantitative analysis of the spectra which demonstrated that the amorphous calcium phosphate formed accounted for all of the calcium and phosphate that was removed from solution in response to calcium addition. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.publisher American Chemical Society en
dc.relation.ispartofseries Journal of Agricultural and Food Chemistry en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://pubs.acs.org/page/copyright/index.html http://www.sherpa.ac.uk/romeo/issn/0021-8561/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Calcium phosphates in Ca(2+)-fortified milk: phase identification and quantification by Raman spectroscopy en
dc.type Journal Article en
dc.identifier.doi 10.1021/jf503602n en
pubs.issue 50 en
pubs.begin-page 12223 en
pubs.volume 62 en
dc.rights.holder Copyright: American Chemical Society en
dc.identifier.pmid 25414967 en
pubs.end-page 12228 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Journal Article en
pubs.subtype Article en
pubs.elements-id 463299 en
pubs.org-id Science en
pubs.org-id Chemistry en
dc.identifier.eissn 1520-5118 en
pubs.record-created-at-source-date 2015-05-06 en
pubs.dimensions-id 25414967 en


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