Efficacy of several candidate protein biomarkers in the differentiation of vaginal from buccal epithelial cells.

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dc.contributor.author Simons, Joanne en
dc.contributor.author Vintiner, SK en
dc.date.accessioned 2015-05-08T03:05:29Z en
dc.date.issued 2012-11 en
dc.identifier.citation Journal of forensic sciences, 2012, 57 (6), pp. 1585 - 1590 en
dc.identifier.issn 0022-1198 en
dc.identifier.uri http://hdl.handle.net/2292/25441 en
dc.description.abstract Currently, there is no accurate method to differentiate vaginal epithelial cells from buccal epithelial cells in biological samples typically encountered in forensic casework. This study tested the expression of a selection of candidate proteins in buccal and vaginal epithelial cells. We investigated six candidate biomarkers, such as loricrin, vimentin, stratifin, cytokeratin 4, cytokeratin 13, small proline-rich protein 2, and involucrin, using Western blot analysis on whole protein extracts and immunohistochemistry (IHC) on intact cells in an attempt to identify cell-specific markers that would differentiate these cells by microscopy. Involucrin, loricrin, and stratifin showed differential expression during Western blot analysis and were carried through to IHC. Although proteins unique to vaginal epithelial cells and buccal epithelial cells were not identified from among the proteins tested, the increased expression levels of two proteins, loricrin and stratifin in vaginal cells, when compared to buccal cells, do provide encouraging results in the search for epithelial cell-specific markers. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.publisher American Academy of Forensic Sciences en
dc.relation.ispartofseries Journal of forensic sciences en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1556-4029/homepage/ForAuthors.html http://www.sherpa.ac.uk/romeo/issn/0022-1198/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Forensic Science en
dc.subject Immunohistochemistry en
dc.subject Western Blot Analysis en
dc.subject Epithelial Cells en
dc.subject Vaginal en
dc.subject Buccal en
dc.subject Biomarkers en
dc.title Efficacy of several candidate protein biomarkers in the differentiation of vaginal from buccal epithelial cells. en
dc.type Journal Article en
dc.identifier.doi 10.1111/j.1556-4029.2012.02158.x en
pubs.issue 6 en
pubs.begin-page 1585 en
pubs.volume 57 en
dc.rights.holder Copyright: American Academy of Forensic Sciences en
dc.identifier.pmid 22612601 en
pubs.author-url http://onlinelibrary.wiley.com/doi/10.1111/j.1556-4029.2012.02158.x/abstract en
pubs.end-page 1590 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Journal Article en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype Article en
pubs.elements-id 466716 en
pubs.org-id Libraries & Learning Services en
pubs.org-id Libraries & Learning Services en
pubs.org-id Research and Collections en
pubs.org-id Research and Collections en
pubs.org-id Research Services en
pubs.org-id Research Services en
dc.identifier.eissn 1556-4029 en
pubs.record-created-at-source-date 2015-05-08 en
pubs.dimensions-id 22612601 en

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