Effect of indomethacin and lactoferrin on human tenocyte proliferation and collagen formation in vitro

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dc.contributor.author Zhang, Y en
dc.contributor.author Wang, X en
dc.contributor.author Qiu, Y en
dc.contributor.author Cornish, Jillian en
dc.contributor.author Carr, AJ en
dc.contributor.author Xia, Z en
dc.date.accessioned 2015-05-14T23:49:16Z en
dc.date.issued 2014-11 en
dc.identifier.citation Biochemical and Biophysical Research Communications, 2014, 454 (2), pp. 301 - 307 en
dc.identifier.issn 1090-2104 en
dc.identifier.uri http://hdl.handle.net/2292/25521 en
dc.description.abstract Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used in patients with injuries and inflammation of tendon and ligament, and as post-surgical analgesics. The aim of this study is to investigate the effect of indomethacin, a classic NSAID and its combinational effect with an anabolic agent of skeletal tissue, lactoferrin, on the proliferation and collagen formation of human tenocytes in vitro. A factorial experimental design was employed to study the dose-dependent effect of the combination of indomethacin and lactoferrin. The results showed that indomethacin at high concentration (100 μM) inhibited human tenocyte proliferation in culture medium with 1-10% fetal bovine serum (FBS) in vitro. Also, high dose of indomethacin inhibited the collagen formation of human tenocytes in 1% FBS culture medium. Lactoferrin at 50-100 μg/ml promoted human tenocyte survival in serum-free culture medium and enhanced proliferation and collagen synthesis of human tenocytes in 1% FBS culture medium. When 50-100 μg/ml lactoferrin was used in combination with 100-200 μM indomethacin, it partially rescued the inhibitory effects of indomethacin on human tenocyte proliferation, viability and collagen formation. To our knowledge, it is the first evidence that lactoferrin is anabolic to human tenocytes in vitro and reverses potential inhibitory effects of NSAIDs on human tenocytes. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.publisher Elsevier en
dc.relation.ispartofseries Biochemical and Biophysical Research Communications en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.elsevier.com/about/policies/article-posting-policy http://www.sherpa.ac.uk/romeo/issn/0006-291X/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Animals en
dc.subject Cattle en
dc.subject Humans en
dc.subject Tendons en
dc.subject Cells, Cultured en
dc.subject Collagen en
dc.subject Indomethacin en
dc.subject Lactoferrin en
dc.subject Anti-Inflammatory Agents, Non-Steroidal en
dc.subject Drug Combinations en
dc.subject Cell Proliferation en
dc.subject Cell Survival en
dc.subject Adult en
dc.subject Anti-Infective Agents en
dc.subject Male en
dc.title Effect of indomethacin and lactoferrin on human tenocyte proliferation and collagen formation in vitro en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.bbrc.2014.10.061 en
pubs.issue 2 en
pubs.begin-page 301 en
pubs.volume 454 en
dc.rights.holder Copyright: Elsevier en
dc.identifier.pmid 25450393 en
pubs.end-page 307 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Journal Article en
pubs.subtype Article en
pubs.elements-id 468553 en
pubs.org-id Medical and Health Sciences en
pubs.org-id School of Medicine en
pubs.org-id Medicine Department en
pubs.org-id Science en
pubs.org-id Science Research en
pubs.org-id Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1090-2104 en
pubs.record-created-at-source-date 2015-05-15 en
pubs.dimensions-id 25450393 en


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