dc.contributor.author |
Lynch, L |
en |
dc.contributor.author |
Gamblin, A |
en |
dc.contributor.author |
Vintiner, S |
en |
dc.contributor.author |
Simons, Joanne |
en |
dc.date.accessioned |
2015-05-20T22:36:38Z |
en |
dc.date.issued |
2015-05 |
en |
dc.identifier.citation |
Forensic Science International: Genetics, 2015, 16 pp. 1 - 7 |
en |
dc.identifier.issn |
1878-0326 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/25590 |
en |
dc.description.abstract |
During the investigation of allegations of sexual assault, samples are frequently encountered that contain DNA from a female and a male donor. These may represent contributions of DNA from the complainant and potentially, the offender. Many semen stained samples successfully undergo DNA analysis and interpretation using a differential extraction method that separates sperm from the epithelial cells present in the stain. However, for those mixed cell samples that contain only epithelial cells, separation of any male cells from female cells is problematic. This paper describes the application of fluorescent in situ hybridisation (FISH) for the gender identification of epithelial cells and subsequent recovery of target cells using laser microdissection (LMD). The profiling results obtained from samples of known cell numbers using the Identifiler™ multiplex at standard 28-cycle PCR conditions and, when cell numbers are low, the SGM Plus™ multiplex at elevated 34-cycle PCR conditions (also known as Low Copy Number DNA analysis (LCN)) are described. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.publisher |
Elsevier |
en |
dc.relation.ispartofseries |
Forensic Science International: Genetics |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.elsevier.com/about/policies/article-posting-policy#accepted-manuscript
http://www.sherpa.ac.uk/romeo/issn/1872-4973/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
en |
dc.title |
STR profiling of epithelial cells identified by X/Y-FISH labelling and laser microdissection using standard and elevated PCR conditions. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.fsigen.2014.10.017 |
en |
pubs.begin-page |
1 |
en |
pubs.volume |
16 |
en |
dc.description.version |
AM - Accepted manuscript |
en |
dc.rights.holder |
Copyright:
Elsevier |
en |
dc.identifier.pmid |
25555139 |
en |
pubs.end-page |
7 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Journal Article |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
472024 |
en |
pubs.org-id |
Libraries & Learning Services |
en |
pubs.org-id |
Libraries & Learning Services |
en |
pubs.org-id |
Research and Collections |
en |
pubs.org-id |
Research and Collections |
en |
pubs.org-id |
Research Services |
en |
pubs.org-id |
Research Services |
en |
dc.identifier.eissn |
1878-0326 |
en |
pubs.record-created-at-source-date |
2015-05-21 |
en |
pubs.dimensions-id |
25555139 |
en |