How long is too long for cerebral cooling after ischemia in fetal sheep?

Show simple item record Davidson, Joanne en Wassink, Guido en Yuill, Caroline en Zhang, FG en Bennet, Laura en Gunn, Alistair en 2015-06-09T02:02:07Z en 2015 en
dc.identifier.citation Journal of Cerebral Blood Flow and Metabolism, 2015, 35 pp. 751 - 758 en
dc.identifier.issn 0271-678X en
dc.identifier.uri en
dc.description.abstract Therapeutic hypothermia can partially reduce long-term death and disability in neonates after hypoxic-ischemic encephalopathy. The aim of this study was to determine whether prolonging the duration of cooling from 3 days to 5 days could further improve outcomes of cerebral ischemia in near-term fetal sheep. Fetal sheep (0.85 gestation) received 30 minutes bilateral carotid artery occlusion followed by 3 days of normothermia (n=8), 3 days of hypothermia (n=8), or 5 days of hypothermia (n=8) started 3 hours after ischemia. Sham controls received sham ischemia followed by normothermia (n=8). Cerebral ischemia was associated with profound loss of electroencephalography power and spectral edge, with greater and more rapid recovery in both hypothermia groups (P<0.05). Ischemia was associated with severe loss of neurons in the cortex, hippocampus and thalamus (P<0.05), with a significant improvement in both hypothermia groups. However, the ischemia-3-day hypothermia group showed greater neuronal survival in the cortex and dentate gyrus compared with ischemia-5-day hypothermia (P<0.05). Ischemia was associated with induction of iba1-positive microglia, which was attenuated in both hypothermia groups (P<0.05). Extending the duration of delayed therapeutic hypothermia from 3 to 5 days did not improve outcomes after severe ischemia, and was associated with reduced neuronal survival in some regions. en
dc.description.uri en
dc.relation.ispartofseries Journal of Cerebral Blood Flow and Metabolism en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.title How long is too long for cerebral cooling after ischemia in fetal sheep? en
dc.type Journal Article en
dc.identifier.doi 10.1038/jcbfm.2014.259 en
pubs.begin-page 751 en
pubs.volume 35 en
dc.description.version AM - Accepted Manuscript en
dc.identifier.pmid 25605291 en en
pubs.end-page 758 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 473867 en Medical and Health Sciences en Medical Sciences en Physiology Division en
dc.identifier.eissn 1559-7016 en
pubs.record-created-at-source-date 2015-01-31 en
pubs.dimensions-id 25605291 en

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