A novel technology using transscleral ultrasound to deliver protein loaded nanoparticles

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dc.contributor.author Huang, Di en
dc.contributor.author Wang, L en
dc.contributor.author Dong, Y en
dc.contributor.author Pan, X en
dc.contributor.author Li, G en
dc.contributor.author Wu, C en
dc.date.accessioned 2015-07-17T04:34:22Z en
dc.date.issued 2014-09 en
dc.identifier.citation European Journal of Pharmaceutics and Biopharmaceutics, 2014, 88 (1), pp. 104 - 115 en
dc.identifier.issn 0939-6411 en
dc.identifier.uri http://hdl.handle.net/2292/26305 en
dc.description.abstract This study was designed to investigate the feasibility of silk fibroin nanoparticles (SFNs) for sustained drug delivery in transscleral ultrasound. Fluorescein isothiocynate labeled bovine serum albumin (FITC-BSA, MW 66.45 kDa) was chosen as a model macromolecular protein drug and SFNs were used as nano-carrier systems suitable for ocular drug delivery. Drug loaded nanoparticles (FITC-BSA-SFNs) were first prepared and characterized. In vitro transscleral study under ultrasound exposure (1 MHz, 0.5 W/cm2, 5 min continuous wave) using isolated sclera of rabbit was performed. The posterior eye segment of rabbit was examined for adverse effect by slit-lamp and histology. It was found that FITC-BSA-SFNs possessed sustained release, bioadhesive, and co-permeation characteristics. The ultrasound application significantly improved the penetration efficiency of FITC-BSA-SFNs as compared with passive delivery, meanwhile caused no damages to the ocular tissue and particles themselves. The distribution profile of SFNs revealed rapid and lasting adhesion on the outer scleral tissues, followed by migration into the interior up to one week after treatment. This research suggested a novel non-invasive transscleral administration of macromolecular protein drugs using SFN carriers combining with ultrasound technology. en
dc.relation.ispartofseries European Journal of Pharmaceutics and Biopharmaceutics en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0939-6411/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title A novel technology using transscleral ultrasound to deliver protein loaded nanoparticles en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.ejpb.2014.04.011 en
pubs.issue 1 en
pubs.begin-page 104 en
pubs.volume 88 en
dc.identifier.pmid 24833007 en
pubs.end-page 115 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 487689 en
pubs.record-created-at-source-date 2015-07-17 en
pubs.dimensions-id 24833007 en

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