dc.contributor.author |
Locke, Michelle |
en |
dc.contributor.author |
Ussher, JE |
en |
dc.contributor.author |
Mistry, R |
en |
dc.contributor.author |
Taylor, John |
en |
dc.contributor.author |
Dunbar, Peter |
en |
dc.date.accessioned |
2015-07-28T23:55:43Z |
en |
dc.date.available |
2011-05-10 |
en |
dc.date.issued |
2011-08-29 |
en |
dc.identifier.citation |
Tissue Engineering Part C: Methods, 2011, 17 (9), pp. 949 - 959 (11) |
en |
dc.identifier.issn |
1937-3384 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/26489 |
en |
dc.description.abstract |
Human adipose-derived stem cells (ASCs) are attractive targets for genetic manipulation and cellular therapies. However, current methods of gene transfer are limited by lack of efficiency, toxicity, or safety concerns. Recombinant adeno-associated virus (rAAV) has been extensively assessed as a gene therapy vector and has an excellent safety profile. This study reports the efficient transduction of well-characterized, homogeneous cultures of human ASCs by rAAV serotypes 2, 5, and 6. Transduction with rAAV2 at high multiplicity of infection was associated with reduced cell viability; however, no adverse effect was seen with serotypes 5 and 6. A further increase in transduction efficiency was observed using a rAAV6 Y731F tyrosine capsid mutant. rAAV-transduced ASCs retained their adipogenic potential. Therefore, rAAV serotypes 2, 5, and 6 should be considered the vectors of choice for genetic manipulation of ASCs. |
en |
dc.description.uri |
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000294701400008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e41486220adb198d0efde5a3b153e7d |
en |
dc.language |
English |
en |
dc.publisher |
Mary Ann Liebert |
en |
dc.relation.ispartofseries |
Tissue Engineering Part C: Methods |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1937-3384/
http://www.liebertpub.com/nv/resources-tools/self-archiving/51/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Science & Technology |
en |
dc.subject |
Life Sciences & Biomedicine |
en |
dc.subject |
Cell & Tissue Engineering |
en |
dc.subject |
Biotechnology & Applied Microbiology |
en |
dc.subject |
Cell Biology |
en |
dc.subject |
BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
en |
dc.subject |
CELL & TISSUE ENGINEERING |
en |
dc.subject |
CELL BIOLOGY |
en |
dc.subject |
GENE-TRANSFER |
en |
dc.subject |
BONE-MARROW |
en |
dc.subject |
VIRAL VECTORS |
en |
dc.subject |
AAV VECTORS |
en |
dc.subject |
ALPHA-V-BETA-5 INTEGRIN |
en |
dc.subject |
PACKAGING CAPACITY |
en |
dc.subject |
MULTILINEAGE CELLS |
en |
dc.subject |
INTEGRATION SITES |
en |
dc.subject |
LARGE-SCALE |
en |
dc.subject |
SEROTYPE 6 |
en |
dc.title |
Transduction of human adipose-derived mesenchymal stem cells by recombinant adeno-associated virus vectors |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1089/ten.tec.2011.0153 |
en |
pubs.issue |
9 |
en |
pubs.begin-page |
949 |
en |
pubs.volume |
17 |
en |
dc.rights.holder |
Copyright:
Mary Ann Liebert |
en |
dc.identifier.pmid |
21563982 |
en |
pubs.author-url |
http://online.liebertpub.com/doi/abs/10.1089/ten.tec.2011.0153 |
en |
pubs.end-page |
959 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.subtype |
Journal |
en |
pubs.elements-id |
228202 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Surgery Department |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Biological Sciences |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1937-3392 |
en |
pubs.record-created-at-source-date |
2015-07-29 |
en |
pubs.dimensions-id |
21563982 |
en |