Measuring the electrical impedance of mouse brain cortex

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Show simple item record Wilson, MT en Elbohouty, M en Lin, Oliver en Voss, LJ en Jones, K en Steyn-Ross, DA en
dc.coverage.spatial Brisbane, Australia en 2015-12-03T02:20:26Z en 2014 en
dc.identifier.citation 18th International Biophysics Congress, 03 Aug2014 - 07 Aug 2014. Aug 2014 en
dc.identifier.uri en
dc.description.abstract We report on an experimental method to measure conductivity of cortical tissue. We use a pair of 5mm diameter Ag/AgCl electrodes in a Perspex sandwich device that can be brought to a distance of 400 microns apart. The apparatus is brought to uniform temperature before use. Electrical impedance of a sample is measured across the frequency range 20 Hz-2.0 MHz with an Agilent 4980A four-point impedance monitor in a shielded room. The equipment has been used to measure the conductivity of mature mouse brain cortex in vitro. Slices 400 microns in thickness are prepared on a vibratome. Slices are bathed in artificial cerebrospinal fluid (ACSF) to keep them alive. Slices are removed from the ACSF and sections of cortical tissue approximately 2 mm times 2 mm are cut with a razor blade. The sections are photographed through a calibrated microscope to allow identification of their cross-sectional areas. Excess ACSF is removed from the sample and the sections places between the electrodes. The impedance is measured across the frequency range and electrical conductivity calculated. Results show two regions of dispersion. A low frequency region is evident below approximately 10 kHz, and a high frequency dispersion above this. Results at the higher frequencies show a good fit to the Cole-Cole model of impedance of biological tissue; this model consists of resistive and non-linear capacitive elements. Physically, these elements are likely to arise due to membrane polarization and migration of ions both intra- and extra-cellularly. en
dc.description.uri en
dc.relation.ispartof 18th International Biophysics Congress en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.title Measuring the electrical impedance of mouse brain cortex en
dc.type Conference Poster en en
dc.rights.accessrights en
pubs.elements-id 505330 en
pubs.record-created-at-source-date 2015-11-18 en

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