Platelet-rich plasma protects tenocytes from adverse side effects of dexamethasone and ciprofloxacin

Show simple item record Zargar Baboldashti, N en Poulsen, Raewyn en Franklin, SL en Thompson, MS en Hulley, PA en 2016-07-22T00:44:04Z en 2011-09-02 en
dc.identifier.citation American Journal of Sports Medicine, 2011, 39 (9), pp. 1929 - 1935 (7) en
dc.identifier.issn 0363-5465 en
dc.identifier.uri en
dc.description.abstract BACKGROUND: Ruptured tendons heal very slowly and complete recovery from injury is uncertain. Platelet-rich plasma (PRP), a rich source of growth factors, is currently being widely tested as a soft tissue healing agent and may accelerate tendon repair. The authors assessed the ability of PRP to prevent in vitro adverse effects of 2 drugs commonly linked to tendon rupture and tendinopathy, glucocorticoids and fluoroquinolone antibiotics. HYPOTHESIS: The pro-healing response induced by PRP protects human tenocytes against the cytotoxic effects of dexamethasone and ciprofloxacin. STUDY DESIGN: Controlled laboratory study. METHODS: Human primary hamstring tenocytes were exposed to different doses of ciprofloxacin and dexamethasone with and without PRP. AlamarBlue, β-galactosidase assay, and live/dead stain were used to measure, respectively, viability, senescence, and death in tenocyte culture. RESULTS: The viability of cells exposed to high doses of ciprofloxacin was significantly decreased compared with controls, with no induced senescence but increased cell death. Dexamethasone reduced viable cell number without inducing overt cell death, but the number of senescent cells increased considerably. After co-treatment with 10% PRP, viable cell number increased significantly in both conditions and the number of dead cells decreased in ciprofloxacin-treated cultures. Moreover, dexamethasone-induced senescence was markedly reduced by co-treatment with 10% PRP. CONCLUSION: This study demonstrates that ciprofloxacin and dexamethasone have differing adverse effects on human tenocytes, with ciprofloxacin inducing cell death while dexamethasone primarily induces senescence. The authors showed that PRP can protect cultured human tenocytes against cell death or senescence induced by these drugs. CLINICAL RELEVANCE: Both ciprofloxacin and dexamethasone are highly effective in treatment of inflammatory and infectious conditions, therefore new strategies to minimize their adverse effects are of strong interest. These findings suggest the potential for local administration of PRP to enhance tendon healing in patients undergoing glucocorticoid or fluoroquinolone treatment. en
dc.description.uri en
dc.format.medium Print-Electronic en
dc.language English en
dc.publisher SAGE Publications (UK and US): No SAGE Choice en
dc.relation.ispartofseries American Journal of Sports Medicine en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.subject Tendons en
dc.subject Cells, Cultured en
dc.subject Humans en
dc.subject Rupture en
dc.subject Tendon Injuries en
dc.subject Ciprofloxacin en
dc.subject Dexamethasone en
dc.subject Glucocorticoids en
dc.subject Anti-Infective Agents en
dc.subject Platelet Transfusion en
dc.subject Wound Healing en
dc.subject Cell Aging en
dc.subject Cell Proliferation en
dc.subject Adult en
dc.subject Middle Aged en
dc.subject Tendinopathy en
dc.subject Platelet-Rich Plasma en
dc.subject Young Adult en
dc.title Platelet-rich plasma protects tenocytes from adverse side effects of dexamethasone and ciprofloxacin en
dc.type Journal Article en
dc.identifier.doi 10.1177/0363546511407283 en
pubs.issue 9 en
pubs.begin-page 1929 en
pubs.volume 39 en
dc.description.version VoR - Version of Record en
dc.rights.holder en
dc.identifier.pmid 21632978 en en
pubs.end-page 1935 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 496437 en Medical and Health Sciences en School of Medicine en Medicine Department en
dc.identifier.eissn 1552-3365 en
pubs.record-created-at-source-date 2016-07-22 en 2011-06-01 en
pubs.dimensions-id 21632978 en

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