HMGB1 Translocation After Ischemia in the Ovine Fetal Brain

Show simple item record Zhang, J en Klufas, D en Manalo, K en Adjepong, K en Davidson, Joanne en Wassink, Guido en Bennet, Laura en Gunn, Alistair en Stopa, EG en Liu, K en Nishibori, M en Stonestreet, BS en 2016-07-22T05:37:28Z en 2016-06 en
dc.identifier.citation Journal of Neuropathology and Experimental Neurology, 2016, 75 (6), pp. 527 - 538 en
dc.identifier.issn 0022-3069 en
dc.identifier.uri en
dc.description.abstract Inflammation contributes to the evolution of hypoxic-ischemic (HI) brain injury. High-mobility group box-1 (HMGB1) is a nuclear protein that is translocated from the nucleus and released after ischemia in adult rodents and thereby initiates inflammatory responses. However, there is very little information regarding the effects of HI on HMGB1 in immature brains. To investigate the effects of HI on HMGB1 in the term-equivalent fetal brain, ovine fetuses at 127 days gestation were studied after 30 minutes of carotid occlusion. Groups were sham-control and ischemia with 48 hours and ischemia with 72 hours of reperfusion. By immunohistochemistry, HMGB1 was found to be localized primarily in cell nuclei and partially in cytoplasmic compartments in the cerebral cortex of controls. Ischemia increased the area fraction of neuronal cells with cytoplasmic HMGB1 staining, and Western immunoblot revealed that cytosolic HMGB1 expression increased after ischemia (p < 0.05) and decreased in nuclei in ischemic versus the sham-control brains (p < 0.05). These data indicate that HMGB1 translocates from the nuclear to cytosolic compartments after ischemic brain injury in fetal sheep. This translocation may enable the action of HMGB1 as a proinflammatory cytokine that contributes to HI injury in the developing brain. en
dc.description.uri en
dc.publisher Oxford University Press (OUP) en
dc.relation.ispartofseries Journal of Neuropathology and Experimental Neurology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.title HMGB1 Translocation After Ischemia in the Ovine Fetal Brain en
dc.type Journal Article en
dc.identifier.doi 10.1093/jnen/nlw030 en
pubs.issue 6 en
pubs.begin-page 527 en
pubs.volume 75 en
dc.rights.holder Copyright: American Association of Neuropathologists, Inc en
dc.identifier.pmid 27151753 en en
pubs.end-page 538 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 527498 en Medical and Health Sciences en Medical Sciences en Physiology Division en
dc.identifier.eissn 1554-6578 en
pubs.record-created-at-source-date 2016-05-09 en
pubs.dimensions-id 27151753 en

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