Uncovering Transgenerational Parent-of-Origin Effects in Caenorhabditis elegans and an Investigation into the Causal Loci of Endoderm Plasticity

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dc.contributor.advisor Rothman, J en
dc.contributor.author Al-Alami, Coco en
dc.date.accessioned 2016-07-28T21:16:45Z en
dc.date.issued 2016 en
dc.identifier.citation 2016 en
dc.identifier.uri http://hdl.handle.net/2292/29659 en
dc.description Full text is available to authenticated members of The University of Auckland only. en
dc.description.abstract Previous investigation into the plasticity of Caenorhabditis elegans (C. elegans) endoderm development had revealed broad variation in the requirement for the key regulatory input, SKN-1. SKN-1 is an essential transcription factor which functions within the endomesoderm gene regulatory network (GRN) to specify the fate of endoderm. Testing the requirement for SKN-1 was achieved via skn-1(RNAi) knock down. An immense amount of variation was seen across all 97 wild-type C. elegans isolates (Yamila Torres Cleuren, personal communication, February 2015), in addition to a strong parent-dependent effect. The research here sought to determine whether parent-of-origin effects (POEs) would be observed when a skn-1 mutant was introgressed into the background of a sample population of wild-type isolates. Backcrossing the skn-1(zu67) mutation from an N2 derived strain, JJ185, into three different wild-type populations was completed through to the F12 generation. Introgressions were completed reciprocally to the F6 generation i.e. the JJ185 hermaphrodite was crossed with a wild-type male and then a cross was initiated between the wild-type hermaphrodites and JJ185 males. POEs were observed between all three different reciprocal events and a variety of potential mechanisms have been proposed that may have led to these effects. In addition an RNAi genetic screen of 13 candidate genes (with previously known function) was performed on skn-1 mutant strains, mom-2 and a sample of the wild-type C. elegans population. The purpose of which, was to determine if any of these genes might account for the variation in SKN-1 dependence that was previously observed. The genetic screen results failed to suggest any causal effect in endoderm development. However, there was evidence to suggest that the candidate gene uba-1 (C47E12.5) affects embryo development differently in different genetic backgrounds. The study of inheritance within C. elegans is an efficient and relatively effective system. The insights gained from these unique model organisms may help in the prediction of genetic risk factors in critical inheritance systems, which could contribute towards the improvement of human health. en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof Masters Thesis - University of Auckland en
dc.relation.isreferencedby UoA99264865410502091 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights Restricted Item. Available to authenticated members of The University of Auckland. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/nz/ en
dc.title Uncovering Transgenerational Parent-of-Origin Effects in Caenorhabditis elegans and an Investigation into the Causal Loci of Endoderm Plasticity en
dc.type Thesis en
thesis.degree.discipline Chemistry en
thesis.degree.grantor The University of Auckland en
thesis.degree.level Masters en
dc.rights.holder Copyright: The Author en
pubs.elements-id 537021 en
pubs.record-created-at-source-date 2016-07-29 en


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