dc.contributor.author |
Owens, L |
en |
dc.contributor.author |
Simanski, S |
en |
dc.contributor.author |
Squire, Christopher |
en |
dc.contributor.author |
Smith, A |
en |
dc.contributor.author |
Cartzendafner, J |
en |
dc.contributor.author |
Cavett, V |
en |
dc.contributor.author |
Caldwell Busby, J |
en |
dc.contributor.author |
Sato, T |
en |
dc.contributor.author |
Ayad, NG |
en |
dc.date.accessioned |
2016-08-09T01:48:50Z |
en |
dc.date.issued |
2010-02 |
en |
dc.identifier.citation |
Journal of Biological Chemistry, 2010, 285 (9), pp. 6761 - 6769 |
en |
dc.identifier.issn |
0021-9258 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/29855 |
en |
dc.description.abstract |
Cell cycle progression is dependent upon coordinate regulation of kinase and proteolytic pathways. Inhibitors of cell cycle transitions are degraded to allow progression into the subsequent cell cycle phase. For example, the tyrosine kinase and Cdk1 inhibitor Wee1 is degraded during G(2) and mitosis to allow mitotic progression. Previous studies suggested that the N terminus of Wee1 directs Wee1 destruction. Using a chemical mutagenesis strategy, we report that multiple regions of Wee1 control its destruction. Most notably, we find that the activation domain of the Wee1 kinase is also required for its degradation. Mutations in this domain inhibit Wee1 degradation in somatic cell extracts and in cells without affecting the overall Wee1 structure or kinase activity. More broadly, these findings suggest that kinase activation domains may be previously unappreciated sites of recognition by the ubiquitin proteasome pathway. |
en |
dc.description.uri |
http://www.jbc.org/ |
en |
dc.publisher |
American Society for Biochemistry and Molecular Biology |
en |
dc.relation.ispartofseries |
Journal of Biological Chemistry |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0021-9258/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Activation Domain-Dependent Degradation of Somatic Wee1 Kinase |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1074/jbc.M109.093237 |
en |
pubs.issue |
9 |
en |
pubs.begin-page |
6761 |
en |
pubs.volume |
285 |
en |
dc.description.version |
VoR – Version of Record |
en |
dc.rights.holder |
Copyright:
American Society for Biochemistry and Molecular Biology |
en |
pubs.author-url |
http://www.jbc.org/content/285/9/6761.full |
en |
pubs.end-page |
6769 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
81586 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Biological Sciences |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1083-351X |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |