Activation Domain-Dependent Degradation of Somatic Wee1 Kinase

Show simple item record Owens, L en Simanski, S en Squire, Christopher en Smith, A en Cartzendafner, J en Cavett, V en Caldwell Busby, J en Sato, T en Ayad, NG en 2016-08-09T01:48:50Z en 2010-02 en
dc.identifier.citation Journal of Biological Chemistry, 2010, 285 (9), pp. 6761 - 6769 en
dc.identifier.issn 0021-9258 en
dc.identifier.uri en
dc.description.abstract Cell cycle progression is dependent upon coordinate regulation of kinase and proteolytic pathways. Inhibitors of cell cycle transitions are degraded to allow progression into the subsequent cell cycle phase. For example, the tyrosine kinase and Cdk1 inhibitor Wee1 is degraded during G(2) and mitosis to allow mitotic progression. Previous studies suggested that the N terminus of Wee1 directs Wee1 destruction. Using a chemical mutagenesis strategy, we report that multiple regions of Wee1 control its destruction. Most notably, we find that the activation domain of the Wee1 kinase is also required for its degradation. Mutations in this domain inhibit Wee1 degradation in somatic cell extracts and in cells without affecting the overall Wee1 structure or kinase activity. More broadly, these findings suggest that kinase activation domains may be previously unappreciated sites of recognition by the ubiquitin proteasome pathway. en
dc.description.uri en
dc.publisher American Society for Biochemistry and Molecular Biology en
dc.relation.ispartofseries Journal of Biological Chemistry en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.title Activation Domain-Dependent Degradation of Somatic Wee1 Kinase en
dc.type Journal Article en
dc.identifier.doi 10.1074/jbc.M109.093237 en
pubs.issue 9 en
pubs.begin-page 6761 en
pubs.volume 285 en
dc.description.version VoR – Version of Record en
dc.rights.holder Copyright: American Society for Biochemistry and Molecular Biology en en
pubs.end-page 6769 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 81586 en Science en Biological Sciences en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1083-351X en
pubs.record-created-at-source-date 2010-09-01 en

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