Sphingosine-1-phosphate lyase is expressed by CD68+ cells on the parenchymal side of marginal reticular cells in human lymph nodes

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dc.contributor.author Park, Saem en
dc.contributor.author Angel, Catherine en
dc.contributor.author McIntosh, Julie en
dc.contributor.author Brooks, Anna en
dc.contributor.author Middleditch, M en
dc.contributor.author Chen, Chun-Jen en
dc.contributor.author Ruggiero, Katya en
dc.contributor.author Cebon, J en
dc.contributor.author Rod Dunbar, P en
dc.date.accessioned 2016-08-17T04:07:47Z en
dc.date.issued 2014-08 en
dc.identifier.citation European Journal of Immunology, 2014, 44(8), pp. 2425 - 2436 en
dc.identifier.issn 1521-4141 en
dc.identifier.uri http://hdl.handle.net/2292/30029 en
dc.description.abstract Lymph nodes (LNs) form the intersection between the vascular and lymphatic systems. Lymphocytes and antigen-presenting cells (APCs) traffic between these systems, but the barriers crossed during this trafficking in human LNs are poorly defined. We identified a population of cells in human LNs that lines the boundary between the parenchyma and lymphatic sinuses, consistent with descriptions of marginal reticular cells (MRCs) in murine LNs. Human MRCs are CD141(high) podoplanin(+), CD90(+), ICAM1(+), and VCAM1(+) but lack endothelial and hematopoietic cell markers, or alpha-smooth muscle actin. We then examined expression of the enzyme sphingosine-1-phosphate (S1P) lyase (SGPL1) relative to the boundary defined by MRCs. SGPL1 expression was almost exclusively restricted to cells on the parenchymal side of MRCs, consistent with a role in maintaining the S1P gradient between the sinuses and the parenchyma. Surprisingly the cells expressing SGPL1 in the parenchyma were CD68(+) APCs. CD68(+) APCs generated from human monocytes were able to internalize and irreversibly degrade S1P, and this activity was inhibited by the S1P analogue FTY720. This work provides a map of the key structures at the boundary where human lymphocytes egress into sinuses, and identifies a novel potential mechanism for the activity of S1P analogues in humans. en
dc.description.uri http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1521-4141/issues en
dc.publisher Wiley-VCH Verlag en
dc.relation.ispartofseries European Journal of Immunology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0014-2980/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject FTY720 en
dc.subject Lymph node en
dc.subject Lymphocyte egress en
dc.subject Marginal reticular cells en
dc.subject Sphingosine 1-phosphate en
dc.subject Aldehyde-Lyases en
dc.subject Antigens, CD en
dc.subject Antigens, Differentiation, Myelomonocytic en
dc.subject Cell Movement en
dc.subject Humans en
dc.subject Lymph Nodes en
dc.subject Lymphatic System en
dc.subject Lymphocytes en
dc.subject Lysophospholipids en
dc.subject Mesophyll Cells en
dc.subject Monocytes en
dc.subject Sphingosine en
dc.title Sphingosine-1-phosphate lyase is expressed by CD68+ cells on the parenchymal side of marginal reticular cells in human lymph nodes en
dc.type Journal Article en
dc.identifier.doi 10.1002/eji.201344158 en
pubs.issue 8 en
pubs.begin-page 2425 en
pubs.volume 44 en
dc.identifier.pmid 24825162 en
pubs.author-url http://onlinelibrary.wiley.com/doi/10.1002/eji.201344158/full en
pubs.end-page 2436 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 439847 en
pubs.org-id Science en
pubs.org-id Biological Sciences en
pubs.org-id Science Research en
pubs.org-id Maurice Wilkins Centre en
pubs.org-id Maurice Wilkins Centre (2010-2014) en
pubs.org-id Statistics en
dc.identifier.eissn 1521-4141 en
pubs.record-created-at-source-date 2016-08-17 en
pubs.dimensions-id 24825162 en

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