Uropathogenic Escherichia coli Releases Extracellular Vesicles That Are Associated with RNA

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dc.contributor.author Blenkiron, Cherie en
dc.contributor.author Simonov, Denis en
dc.contributor.author Muthukaruppan, Anitadevi en
dc.contributor.author Tsai, Peter en
dc.contributor.author Dauros Singorenko, Priscila en
dc.contributor.author Green, S en
dc.contributor.author Hong, Jiwon en
dc.contributor.author Print, Cristin en
dc.contributor.author Swift, Simon en
dc.contributor.author Phillips, Anthony en
dc.contributor.editor Cascales, E en
dc.date.accessioned 2016-10-06T04:42:31Z en
dc.date.issued 2016-08-08 en
dc.identifier.citation PLoS One 11(8) Article number e0160440 08 Aug 2016 en
dc.identifier.issn 1932-6203 en
dc.identifier.uri http://hdl.handle.net/2292/30645 en
dc.description.abstract BACKGROUND: Bacterium-to-host signalling during infection is a complex process involving proteins, lipids and other diffusible signals that manipulate host cell biology for pathogen survival. Bacteria also release membrane vesicles (MV) that can carry a cargo of effector molecules directly into host cells. Supported by recent publications, we hypothesised that these MVs also associate with RNA, which may be directly involved in the modulation of the host response to infection. METHODS AND RESULTS: Using the uropathogenic Escherichia coli (UPEC) strain 536, we have isolated MVs and found they carry a range of RNA species. Density gradient centrifugation further fractionated and characterised the MV preparation and confirmed that the isolated RNA was associated with the highest particle and protein containing fractions. Using a new approach, RNA-sequencing of libraries derived from three different 'size' RNA populations (<50nt, 50-200nt and 200nt+) isolated from MVs has enabled us to now report the first example of a complete bacterial MV-RNA profile. These data show that MVs carry rRNA, tRNAs, other small RNAs as well as full-length protein coding mRNAs. Confocal microscopy visualised the delivery of lipid labelled MVs into cultured bladder epithelial cells and showed their RNA cargo labelled with 5-EU (5-ethynyl uridine), was transported into the host cell cytoplasm and nucleus. MV RNA uptake by the cells was confirmed by droplet digital RT-PCR of csrC. It was estimated that 1% of MV RNA cargo is delivered into cultured cells. CONCLUSIONS: These data add to the growing evidence of pathogenic bacterial MV being associated a wide range of RNAs. It further raises the plausibility for MV-RNA-mediated cross-kingdom communication whereby they influence host cell function during the infection process. en
dc.publisher Public Library of Science en
dc.relation.ispartofseries PLoS One en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1932-6203/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.title Uropathogenic Escherichia coli Releases Extracellular Vesicles That Are Associated with RNA en
dc.type Journal Article en
dc.identifier.doi 10.1371/journal.pone.0160440 en
pubs.issue 8 en
pubs.volume 11 en
dc.description.version VoR - Version of Record en
dc.rights.holder Copyright: The authors en
dc.identifier.pmid 27500956 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Article en
pubs.elements-id 538973 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Molecular Medicine en
pubs.org-id School of Medicine en
pubs.org-id Obstetrics and Gynaecology en
pubs.org-id Science en
pubs.org-id Biological Sciences en
pubs.org-id Science Research en
pubs.org-id Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1932-6203 en
pubs.number e0160440 en
pubs.record-created-at-source-date 2016-10-06 en
pubs.online-publication-date 2016-08-08 en
pubs.dimensions-id 27500956 en

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