dc.contributor.author |
Yao, Xudong |
en |
dc.contributor.author |
Bunt, C |
en |
dc.contributor.author |
Cornish, Jillian |
en |
dc.contributor.author |
Quek, Siew-Young |
en |
dc.contributor.author |
Wen, Jingyuan |
en |
dc.date.accessioned |
2016-10-07T02:22:32Z |
en |
dc.date.issued |
2014-12 |
en |
dc.identifier.citation |
Chemical Biology and Drug Design, 2014, 84 (6), pp. 676 - 684 |
en |
dc.identifier.issn |
1747-0277 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/30659 |
en |
dc.description.abstract |
Oral delivery is the most common method for bovine lactoferrin (bLf) administration. However, the presence of proteolytic enzymes in the stomach and intestine limits the effective absorption of bLf within the gastrointestinal (GI) tract. To determine the extent of bLf proteolysis, several digestion models were developed using luminal extracts and mucosal homogenates isolated from four regions of rat intestine: duodenum, jejunum, ileum, and proximal colon. The kinetics of bLf degradation followed a pseudo-first-order rate, and almost complete hydrolysis of bLf was observed in the luminal extracts, indicating that bLf is more susceptive to luminal peptidases rather than mucosal enzymes. Moreover, a significant reduction in bLf proteolysis was observed in the presence of soybean trypsin inhibitor (SBTI), bestatin, and bacitracin, suggesting that there exist trypsin-like and aminopeptidase-like proteases, which play a key role in the degradation of bLf in the intestine. Lactoferrin was then encapsulated in several lipid-based delivery systems including liposomes and solid lipid particles (SLPs) with polymer modification, showing at least 50% of intact bLf remaining after 6 h of digestion compared with native bLf. These findings suggest that particle encapsulation may modulate protein digestion and possibly achieve sufficient oral bioavailability of bLf. |
en |
dc.description.uri |
http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1747-0285 |
en |
dc.publisher |
Wiley |
en |
dc.relation.ispartofseries |
Chemical Biology and Drug Design |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1747-0277/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
bovine lactoferrin |
en |
dc.subject |
enzyme inhibitor |
en |
dc.subject |
particulate delivery system |
en |
dc.subject |
proteolysis |
en |
dc.subject |
pseudo-first-order rate |
en |
dc.subject |
Administration, Oral |
en |
dc.subject |
Animals |
en |
dc.subject |
Cattle |
en |
dc.subject |
Chromatography, High Pressure Liquid |
en |
dc.subject |
Enzyme Inhibitors |
en |
dc.subject |
Intestinal Mucosa |
en |
dc.subject |
Intestines |
en |
dc.subject |
Kinetics |
en |
dc.subject |
Lactoferrin |
en |
dc.subject |
Male |
en |
dc.subject |
Protein Stability |
en |
dc.subject |
Proteolysis |
en |
dc.subject |
Rats |
en |
dc.subject |
Rats, Wistar |
en |
dc.title |
Stability of Bovine Lactoferrin in Luminal Extracts and Mucosal Homogenates from Rat Intestine: A Prelude to Oral Absorption |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1111/cbdd.12360 |
en |
pubs.issue |
6 |
en |
pubs.begin-page |
676 |
en |
pubs.volume |
84 |
en |
dc.rights.holder |
Copyright: Wiley |
en |
dc.identifier.pmid |
24890384 |
en |
pubs.author-url |
http://onlinelibrary.wiley.com/doi/10.1111/cbdd.12360/full |
en |
pubs.end-page |
684 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
440864 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Pharmacy |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Medicine Department |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Chemistry |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1747-0285 |
en |
pubs.record-created-at-source-date |
2016-10-07 |
en |
pubs.dimensions-id |
24890384 |
en |