Stability of Bovine Lactoferrin in Luminal Extracts and Mucosal Homogenates from Rat Intestine: A Prelude to Oral Absorption

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dc.contributor.author Yao, Xudong en
dc.contributor.author Bunt, C en
dc.contributor.author Cornish, Jillian en
dc.contributor.author Quek, Siew-Young en
dc.contributor.author Wen, Jingyuan en
dc.date.accessioned 2016-10-07T02:22:32Z en
dc.date.issued 2014-12 en
dc.identifier.citation Chemical Biology and Drug Design, 2014, 84 (6), pp. 676 - 684 en
dc.identifier.issn 1747-0277 en
dc.identifier.uri http://hdl.handle.net/2292/30659 en
dc.description.abstract Oral delivery is the most common method for bovine lactoferrin (bLf) administration. However, the presence of proteolytic enzymes in the stomach and intestine limits the effective absorption of bLf within the gastrointestinal (GI) tract. To determine the extent of bLf proteolysis, several digestion models were developed using luminal extracts and mucosal homogenates isolated from four regions of rat intestine: duodenum, jejunum, ileum, and proximal colon. The kinetics of bLf degradation followed a pseudo-first-order rate, and almost complete hydrolysis of bLf was observed in the luminal extracts, indicating that bLf is more susceptive to luminal peptidases rather than mucosal enzymes. Moreover, a significant reduction in bLf proteolysis was observed in the presence of soybean trypsin inhibitor (SBTI), bestatin, and bacitracin, suggesting that there exist trypsin-like and aminopeptidase-like proteases, which play a key role in the degradation of bLf in the intestine. Lactoferrin was then encapsulated in several lipid-based delivery systems including liposomes and solid lipid particles (SLPs) with polymer modification, showing at least 50% of intact bLf remaining after 6 h of digestion compared with native bLf. These findings suggest that particle encapsulation may modulate protein digestion and possibly achieve sufficient oral bioavailability of bLf. en
dc.description.uri http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1747-0285 en
dc.publisher Wiley en
dc.relation.ispartofseries Chemical Biology and Drug Design en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1747-0277/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject bovine lactoferrin en
dc.subject enzyme inhibitor en
dc.subject particulate delivery system en
dc.subject proteolysis en
dc.subject pseudo-first-order rate en
dc.subject Administration, Oral en
dc.subject Animals en
dc.subject Cattle en
dc.subject Chromatography, High Pressure Liquid en
dc.subject Enzyme Inhibitors en
dc.subject Intestinal Mucosa en
dc.subject Intestines en
dc.subject Kinetics en
dc.subject Lactoferrin en
dc.subject Male en
dc.subject Protein Stability en
dc.subject Proteolysis en
dc.subject Rats en
dc.subject Rats, Wistar en
dc.title Stability of Bovine Lactoferrin in Luminal Extracts and Mucosal Homogenates from Rat Intestine: A Prelude to Oral Absorption en
dc.type Journal Article en
dc.identifier.doi 10.1111/cbdd.12360 en
pubs.issue 6 en
pubs.begin-page 676 en
pubs.volume 84 en
dc.rights.holder Copyright: Wiley en
dc.identifier.pmid 24890384 en
pubs.author-url http://onlinelibrary.wiley.com/doi/10.1111/cbdd.12360/full en
pubs.end-page 684 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 440864 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Pharmacy en
pubs.org-id School of Medicine en
pubs.org-id Medicine Department en
pubs.org-id Science en
pubs.org-id Chemistry en
pubs.org-id Science Research en
pubs.org-id Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1747-0285 en
pubs.record-created-at-source-date 2016-10-07 en
pubs.dimensions-id 24890384 en


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