dc.contributor.author |
Lim, Julie |
en |
dc.contributor.author |
Vaghefi Rezaei, Seyed |
en |
dc.contributor.author |
Li, B |
en |
dc.contributor.author |
Nye-Wood, Mitchell |
en |
dc.contributor.author |
Donaldson, Paul |
en |
dc.date.accessioned |
2016-10-20T21:08:03Z |
en |
dc.date.issued |
2016-04 |
en |
dc.identifier.citation |
Investigative Ophthalmology & Visual Science, 2016, 57(4), pp. 1961-1973 |
en |
dc.identifier.issn |
0146-0404 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/30831 |
en |
dc.description.abstract |
Purpose: To assess the morphologic, biochemical, and optical properties of bovine lenses treated with hyperbaric oxygen. Methods: Lenses were exposed to hyperbaric nitrogen (HBN) or hyperbaric oxygen (HBO) for 5 or 15 hours, lens transparency was assessed using bright field microscopy and lens morphology was visualized using confocal microscopy. Lenses were dissected into the outer cortex, inner cortex, and core, and glutathione (GSH) and malondialdehyde (MDA) measured. Gel electrophoresis and Western blotting were used to detect high molecular weight aggregates (HMW) and glutathione mixed protein disulfides (PSSG). T2-weighted MRI was used to measure lens geometry and map the water/protein ratio to allow gradient refractive index (GRIN) profiles to be calculated. Optical modeling software calculated the change in lens optical power, and an anatomically correct model of the light pathway of the bovine eye was used to determine the effects of HBN and HBO on focal length and overall image quality. Results: Lenses were transparent and lens morphology similar between HBN- and HBO-treated lenses. At 5- and 15-hour HBO exposure, GSH and GSSG were depleted and MDA increased in the core. Glutathione mixed protein disulfides were detected in the outer and inner cortex only with no appearance of HMW. Optical changes were detectable only with 15-hour HBO treatment with a decrease in the refractive index of the core, slightly reduced lens thickness, and an increase in optimal focal length, consistent with a hyperopic shift. Conclusions: This system may serve as a model to study changes that occur with advanced aging rather than nuclear cataract formation per se. |
en |
dc.description.uri |
http://iovs.arvojournals.org/ |
en |
dc.publisher |
Association for Research in Vision and Ophthalmology (ARVO) |
en |
dc.relation.ispartofseries |
Investigative Ophthalmology & Visual Science |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0146-0404/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
en |
dc.title |
Characterization of the Effects of Hyperbaric Oxygen on the Biochemical and Optical Properties of the Bovine Lens |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1167/iovs.16-19142 |
en |
pubs.issue |
4 |
en |
pubs.begin-page |
1961 |
en |
pubs.volume |
57 |
en |
dc.description.version |
VoR – Version of Record |
en |
dc.identifier.pmid |
27096754 |
en |
pubs.author-url |
http://iovs.arvojournals.org/article.aspx?articleid=2516673 |
en |
pubs.end-page |
1973 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
526976 |
en |
pubs.org-id |
Academic Services |
en |
pubs.org-id |
Examinations |
en |
pubs.org-id |
Bioengineering Institute |
en |
pubs.org-id |
ABI Associates |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Physiology Division |
en |
pubs.org-id |
Optometry and Vision Science |
en |
dc.identifier.eissn |
1552-5783 |
en |
pubs.record-created-at-source-date |
2016-10-21 |
en |
pubs.dimensions-id |
27096754 |
en |