Characterization of the Effects of Hyperbaric Oxygen on the Biochemical and Optical Properties of the Bovine Lens

Show simple item record Lim, Julie en Vaghefi Rezaei, Seyed en Li, B en Nye-Wood, Mitchell en Donaldson, Paul en 2016-10-20T21:08:03Z en 2016-04 en
dc.identifier.citation Investigative Ophthalmology & Visual Science, 2016, 57(4), pp. 1961-1973 en
dc.identifier.issn 0146-0404 en
dc.identifier.uri en
dc.description.abstract Purpose: To assess the morphologic, biochemical, and optical properties of bovine lenses treated with hyperbaric oxygen. Methods: Lenses were exposed to hyperbaric nitrogen (HBN) or hyperbaric oxygen (HBO) for 5 or 15 hours, lens transparency was assessed using bright field microscopy and lens morphology was visualized using confocal microscopy. Lenses were dissected into the outer cortex, inner cortex, and core, and glutathione (GSH) and malondialdehyde (MDA) measured. Gel electrophoresis and Western blotting were used to detect high molecular weight aggregates (HMW) and glutathione mixed protein disulfides (PSSG). T2-weighted MRI was used to measure lens geometry and map the water/protein ratio to allow gradient refractive index (GRIN) profiles to be calculated. Optical modeling software calculated the change in lens optical power, and an anatomically correct model of the light pathway of the bovine eye was used to determine the effects of HBN and HBO on focal length and overall image quality. Results: Lenses were transparent and lens morphology similar between HBN- and HBO-treated lenses. At 5- and 15-hour HBO exposure, GSH and GSSG were depleted and MDA increased in the core. Glutathione mixed protein disulfides were detected in the outer and inner cortex only with no appearance of HMW. Optical changes were detectable only with 15-hour HBO treatment with a decrease in the refractive index of the core, slightly reduced lens thickness, and an increase in optimal focal length, consistent with a hyperopic shift. Conclusions: This system may serve as a model to study changes that occur with advanced aging rather than nuclear cataract formation per se. en
dc.description.uri en
dc.publisher Association for Research in Vision and Ophthalmology (ARVO) en
dc.relation.ispartofseries Investigative Ophthalmology & Visual Science en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
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dc.title Characterization of the Effects of Hyperbaric Oxygen on the Biochemical and Optical Properties of the Bovine Lens en
dc.type Journal Article en
dc.identifier.doi 10.1167/iovs.16-19142 en
pubs.issue 4 en
pubs.begin-page 1961 en
pubs.volume 57 en
dc.description.version VoR – Version of Record en
dc.identifier.pmid 27096754 en en
pubs.end-page 1973 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 526976 en Academic Services en Examinations en Bioengineering Institute en ABI Associates en Medical and Health Sciences en Medical Sciences en Physiology Division en Optometry and Vision Science en
dc.identifier.eissn 1552-5783 en
pubs.record-created-at-source-date 2016-10-21 en
pubs.dimensions-id 27096754 en

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