dc.contributor.author |
Hackler, L |
en |
dc.contributor.author |
Masuda, T |
en |
dc.contributor.author |
Oliver, Verity |
en |
dc.contributor.author |
Merbs, SL |
en |
dc.contributor.author |
Zack, DJ |
en |
dc.contributor.editor |
Wang, SZ |
en |
dc.date.accessioned |
2015-09-04T04:53:11Z |
en |
dc.date.accessioned |
2016-10-31T00:59:52Z |
en |
dc.date.issued |
2012 |
en |
dc.identifier.citation |
In Retinal Development. Editors: Wang SZ. 884: 289-304. Humana Press 10 May 2012 |
en |
dc.identifier.isbn |
1617798479 |
en |
dc.identifier.isbn |
9781617798481 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/30902 |
en |
dc.description.abstract |
Laser capture microdissection (LCM) is a useful method to isolate specific cells or cell layers of interest from heterogeneous tissues, such as the retina. The collected cells can be used for DNA, RNA, or protein analysis. We have applied LCM technology to isolate cells from the outer nuclear, inner nuclear, and ganglion cell layers of the retina for mRNA and microRNA (miRNA) expression and epigenetic (DNA methylation) analysis. Here, we describe the methods we have employed for sample preparation, LCM-based isolation of retinal layers, RNA/DNA extraction, RNA quality check, microRNA analysis by quantitative PCR, and DNA methylation analysis by bisulfite sequencing. |
en |
dc.description.uri |
http://librarysearch.auckland.ac.nz/UOA2_A:Combined_Local:uoa_alma21145394280002091 |
en |
dc.publisher |
Humana Press |
en |
dc.relation.ispartof |
Retinal development: Methods and protocols |
en |
dc.relation.ispartofseries |
Methods in molecular biology |
en |
dc.relation.replaces |
http://hdl.handle.net/2292/26882 |
en |
dc.relation.replaces |
2292/26882 |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Use of laser capture microdissection for analysis of retinal mRNA/miRNA expression and DNA methylation |
en |
dc.type |
Book Item |
en |
dc.identifier.doi |
10.1007/978-1-61779-848-1_21 |
en |
pubs.begin-page |
289 |
en |
pubs.volume |
884 |
en |
dc.rights.holder |
Copyright:
Humana Press |
en |
pubs.author-url |
http://link.springer.com/protocol/10.1007/978-1-61779-848-1_21 |
en |
pubs.end-page |
304 |
en |
pubs.place-of-publication |
New York, NY |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.elements-id |
495332 |
en |
pubs.number |
21 |
en |
pubs.record-created-at-source-date |
2015-11-17 |
en |
pubs.online-publication-date |
2012-05-10 |
en |