Comparison of pulsatile vs. continuous administration of human placental growth hormone in female C57BL/6J mice

Show simple item record Liao, S en Vickers, Mark en Evans, A en Stanley, Joanna en Baker, Philip en Perry, Johanna en 2016-11-06T23:08:06Z en 2016-10 en
dc.identifier.citation Endocrine, 2016, 54(1), pp. 169-181 en
dc.identifier.issn 1355-008X en
dc.identifier.uri en
dc.description.abstract Exogenous growth hormone has different actions depending on the method of administration. However, the effects of different modes of administration of the placental variant of growth hormone on growth, body composition and glucose metabolism have not been investigated. In this study, we examined the effect of pulsatile vs. continuous administration of recombinant variant of growth hormone in a normal mouse model. Female C57BL/6J mice were randomized to receive vehicle or variant of growth hormone (2 or 5 mg/kg per day) by daily subcutaneous injection (pulsatile) or osmotic pump for 6 days. Pulsatile treatment with 2 and 5 mg/kg per day significantly increased body weight. There was also an increase in liver, kidney and spleen weight via pulsatile treatment, whereas continuous treatment did not affect body weight or organ size. Pulsatile treatment with 5 mg/kg per day significantly increased fasting plasma insulin concentration, whereas with continuous treatment, fasting insulin concentration was not significantly different from the vehicle-treated control. However, a dose-dependent increase in fasting insulin concentration and decrease in insulin sensitivity, as assessed by HOMA, was observed with both modes of treatment. At 5 mg/kg per day, hepatic growth hormone receptor expression was increased compared to vehicle-treated animals, by both modes of administration. Pulsatile variant of growth hormone did not alter the plasma insulin-like growth factor-1 concentration, whereas a slight decrease was observed with continuous variant of growth hormone treatment. Neither pulsatile nor continuous treatment affected hepatic insulin-like growth factor-1 mRNA expression. Our findings suggest that pulsatile variant of growth hormone treatment was more effective in stimulating growth but caused marked hyperinsulinemia in mice. en
dc.description.uri en
dc.publisher Springer en
dc.relation.ispartofseries Endocrine en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.title Comparison of pulsatile vs. continuous administration of human placental growth hormone in female C57BL/6J mice en
dc.type Journal Article en
dc.identifier.doi 10.1007/s12020-016-1060-0 en
pubs.issue 1 en
pubs.begin-page 169 en
pubs.volume 54 en
dc.rights.holder Copyright: Springer en
dc.identifier.pmid 27515803 en en
pubs.end-page 181 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 539993 en Liggins Institute en
dc.identifier.eissn 1559-0100 en
pubs.record-created-at-source-date 2016-11-07 en
pubs.dimensions-id 27515803 en

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