dc.contributor.author |
Rustenhoven, Justin |
en |
dc.contributor.author |
Park, In |
en |
dc.contributor.author |
Schweder, Patrick |
en |
dc.contributor.author |
Scotter, J |
en |
dc.contributor.author |
Correia, J |
en |
dc.contributor.author |
Smith, AM |
en |
dc.contributor.author |
Gibbons, HM |
en |
dc.contributor.author |
Oldfield, RL |
en |
dc.contributor.author |
Bergin, PS |
en |
dc.contributor.author |
Mee, EW |
en |
dc.contributor.author |
Faull, Richard |
en |
dc.contributor.author |
Curtis, Maurice |
en |
dc.contributor.author |
Scott Graham, E |
en |
dc.contributor.author |
Dragunow, Michael |
en |
dc.date.accessioned |
2016-12-07T21:35:29Z |
en |
dc.date.issued |
2016-01-18 |
en |
dc.identifier.citation |
Scientific Reports 6 Article number 19371 18 Jan 2016 |
en |
dc.identifier.issn |
2045-2322 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/31257 |
en |
dc.description.abstract |
Microglia, the resident macrophages of the central nervous system play vital roles in brain homeostasis through clearance of pathogenic material. Microglia are also implicated in neurological disorders through uncontrolled activation and inflammatory responses. To date, the vast majority of microglial studies have been performed using rodent models. Human microglia differ from rodent counterparts in several aspects including their response to pharmacological substances and their inflammatory secretions. Such differences highlight the need for studies on primary adult human brain microglia and methods to isolate them are therefore required. Our procedure generates microglial cultures of >95% purity from both biopsy and autopsy human brain tissue using a very simple media-based culture procedure that takes advantage of the adherent properties of these cells. Microglia obtained in this manner can be utilised for research within a week. Isolated microglia demonstrate phagocytic ability and respond to inflammatory stimuli and their purity makes them suitable for numerous other forms of in vitro studies, including secretome and transcriptome analysis. Furthermore, this protocol allows for the simultaneous isolation of neural precursor cells during the microglial isolation procedure. As human brain tissue is such a precious and valuable resource the simultaneous isolation of multiple cell types is highly beneficial. |
en |
dc.format.medium |
Electronic |
en |
dc.language |
eng |
en |
dc.publisher |
Nature Publishing Group: Open Access Journals - Option C |
en |
dc.relation.ispartofseries |
Scientific Reports |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/2045-2322/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
en |
dc.title |
Isolation of highly enriched primary human microglia for functional studies |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1038/srep19371 |
en |
pubs.volume |
6 |
en |
dc.description.version |
VoR - Version of Record |
en |
dc.identifier.pmid |
26778406 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
517679 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Anatomy and Medical Imaging |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
Pharmacology |
en |
dc.identifier.eissn |
2045-2322 |
en |
pubs.number |
19371 |
en |
pubs.record-created-at-source-date |
2016-12-08 |
en |
pubs.dimensions-id |
26778406 |
en |