In vitro maturation of round spermatids using porcine oviduct epithelial cell monolayer condition medium

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dc.contributor.author Jabed, Anower en
dc.contributor.author Kamal, Tania en
dc.contributor.author Lee, S-M en
dc.contributor.author Kim, BK en
dc.date.accessioned 2017-01-23T03:51:51Z en
dc.date.issued 2005 en
dc.identifier.citation Reproductive and Developmental Biology, 2005, 29 (4), 241 - 245 en
dc.identifier.issn 1738-2432 en
dc.identifier.uri http://hdl.handle.net/2292/31648 en
dc.description.abstract Porcine oviduct epithelial cells (POEC) are widely used in co-culture experiments to improve early embryonic development, in vitro fertilization in embryo transfer programs for domestic animals and in vitro maturation of immature germ cells. POEC were mechanically isolated and cultured in tissue culture medium 199. Cells grew continuously, and confluent monolayers were formed after 7 days. After forming confluent monolayer of epithelial cells, supernatant was collected as the condition medium for maturing round spermatids in vitro. Round spermatids were also separated mechanically and cultured in the POEC condition medium. In this study we observed that 20% of round spermatid cultured were matured into elongating spermatid after 24 h, and about 10% of round spermatid cultured showed complete elongation (elongated spermatid) within 24~48 h of in vitro culture. No further development was observed within 50~72 h and transformed cells lost their viability after 72 h. These preliminary findings suggest that the condition medium from POEC may be possible to overcome the round spermatid block by improving the milieu of culture system. en
dc.language English en
dc.publisher Korean Society of Animal Reproduction en
dc.relation.ispartofseries Reproductive and Developmental Biology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.rdb.or.kr/ForContributors/01.php en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title In vitro maturation of round spermatids using porcine oviduct epithelial cell monolayer condition medium en
dc.type Journal Article en
pubs.issue 4 en
pubs.begin-page 241 en
pubs.volume 29 en
dc.description.version VoR - Version of Record en
pubs.author-url http://www.rdb.or.kr/AboutUs/04.php en
pubs.end-page 245 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 546912 en
pubs.org-id Academic Services en
pubs.org-id Examinations en
dc.identifier.eissn 2288-0151 en
pubs.record-created-at-source-date 2016-11-23 en


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