Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis

Show simple item record Chakraborty, Mandira en Hickey, Anthony en Petrov, Maxim en Macdonald, JR en Thompson, N en Newby, L en Sim, D en Windsor, John en Phillips, Anthony en 2017-02-09T00:11:19Z en 2016-06-24 en 2016-09 en
dc.identifier.citation Pancreatology, September 2016, 16 (5), 739 - 747 en
dc.identifier.issn 1424-3903 en
dc.identifier.uri en
dc.description.abstract Background/objectives Mitochondrial dysfunction occurs in vital organs in experimental acute pancreatitis (AP) and may play an important role in determining severity of AP. However, obtaining vital organ biopsies to measure mitochondrial function (MtF) in patients with AP poses considerable risk of harm. Being able to measure MtF from peripheral blood will bypass this problem. Furthermore, whether mitochondrial dysfunction is detectable in peripheral blood in mild AP is unknown. Therefore, the objective was to evaluate peripheral blood MtF in experimental and clinical AP. Method Mitochondrial respiration was measured using high resolution oxygraphy in an experimental study in caerulein induced AP and in a separate study, in patients with mild AP. Superoxide, cytochrome c, mitochondrial membrane potential (ΔΨ) and adenine triphosphate (ATP) were also measured as other markers of MtF. Results Even though some states of mitochondrial respiration were increased in both experimental and clinical AP, this did not lead to an increase in net ATP in patients with AP. The increased leak respiration in both studies was further proof of dyscoupled mitochondria. In the clinical study there were also features of mitochondrial dysfunction with increased leak flux control ratio, superoxide, ΔΨ and decreased cytochrome c. Conclusion There is evidence of mitochondrial dysfunction with dyscoupled mitochondria, increased superoxide and decreased cytochrome c in patients with mild acute pancreatitis. Further studies should now determine whether mitochondrial function alters with severity in AP and whether mitochondrial dysfunction responds to treatments. en
dc.description.uri en
dc.language English en
dc.publisher Elsevier en
dc.relation.ispartofseries Pancreatology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.title Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.pan.2016.06.659 en
pubs.issue 5 en
pubs.begin-page 739 en
pubs.volume 16 en
dc.description.version VoR - Version of Record en
dc.identifier.pmid 27473495 en en
pubs.end-page 747 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 537392 en Medical and Health Sciences en School of Medicine en Surgery Department en Science en Biological Sciences en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1424-3911 en
pubs.record-created-at-source-date 2017-02-09 en 2016-06-27 en
pubs.dimensions-id 27473495 en

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