Testing neocortical slice viability in non-perfused no-magnesium artificial cerebrospinal fluid solutions

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dc.contributor.author Voss, LJ en
dc.contributor.author George, SA en
dc.contributor.author Sleigh, James en
dc.coverage.spatial Netherlands en
dc.date.accessioned 2017-03-03T01:55:12Z en
dc.date.available 2011-12-01 en
dc.date.issued 2012-03-15 en
dc.identifier.citation Journal of Neuroscience Methods, 15 March 2012, 204 (2), 273 - 275 en
dc.identifier.issn 0165-0270 en
dc.identifier.uri http://hdl.handle.net/2292/32041 en
dc.description.abstract The acute in vitro brain slice model is a widely used neurophysiological research tool. When applying this method, most researchers continuously perfuse slices with carbogenated artificial cerebrospinal fluid (ACSF) to maintain pH balance and tissue oxygen delivery. Common wisdom suggests that static recordings are incompatible with submerged bath methodology because of deficiency in tissue oxygen supply. However, to our knowledge this has not been tested. In this study, we wanted to determine whether neocortical mouse slice viability could be maintained in the medium term (up to 2h) in a shallow, submerged recording bath under non-perfused, static conditions. Seizure-like events (SLEs) were generated in the slices utilizing no-magnesium ACSF and recorded for 2h under three conditions: (1) perfused ACSF condition (n=8), where slices were perfused continuously with carbogenated no-magnesium ACSF; (2) static ACSF condition (n=12), where slices were recorded in pre-carbogenated, but non-perfused (static) no-magnesium ACSF; and (3) static HEPES ACSF condition (n=12), where slices were recorded in non-perfused (static) no-magnesium ACSF with no pre-carbogenation but buffered with HEPES. SLE activity was stable for 2h across all three conditions. There was no statistically significant difference in SLE frequency, amplitude or length between static and perfused conditions. SLE frequency and amplitude were generally lower in the static HEPES buffer condition. The data indicate that robust and stable neocortical SLE activity can be generated for at least 2h in a submersion bath without ACSF perfusion if pH is adequately controlled. en
dc.description.uri https://www.ncbi.nlm.nih.gov/pubmed/22178217 en
dc.language English en
dc.publisher Elsevier en
dc.relation.ispartofseries Journal of Neuroscience Methods en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0165-0270/ https://www.elsevier.com/about/company-information/policies/sharing en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Action Potentials en
dc.subject Animals en
dc.subject Carbon Dioxide en
dc.subject Cerebrospinal Fluid en
dc.subject Female en
dc.subject Hydrogen-Ion Concentration en
dc.subject Magnesium en
dc.subject Male en
dc.subject Mice en
dc.subject Mice, Inbred C57BL en
dc.subject Neocortex en
dc.subject Oxygen en
dc.subject Perfusion en
dc.subject Seizures en
dc.subject Time Factors en
dc.subject Tissue Culture Techniques en
dc.title Testing neocortical slice viability in non-perfused no-magnesium artificial cerebrospinal fluid solutions en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.jneumeth.2011.12.002 en
pubs.issue 2 en
pubs.begin-page 273 en
pubs.volume 204 en
dc.description.version VoR - Version of Record en
dc.identifier.pmid 22178217 en
pubs.author-url http://www.sciencedirect.com/science/article/pii/S0165027011007102 en
pubs.end-page 275 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 265691 en
pubs.org-id Medical and Health Sciences en
pubs.org-id School of Medicine en
pubs.org-id Anaesthesiology en
dc.identifier.eissn 1872-678X en
dc.identifier.pii S0165-0270(11)00710-2 en
pubs.record-created-at-source-date 2017-03-03 en
pubs.dimensions-id 22178217 en

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