Impedance technology reduces the enumeration time of Brettanomyces yeast during beer fermentation

Show simple item record Van Wyk, Sanelle en Oliveira, Maria en 2017-04-30T23:01:12Z en 2016-12 en
dc.identifier.citation Biotechnology Journal 11(12):1667-1672 Dec 2016 en
dc.identifier.issn 1860-6768 en
dc.identifier.uri en
dc.description.abstract Brettanomyces yeasts are increasingly being used to produce lambic style beers and craft beers with unique flavors. Currently, the industry monitors Brettanomyces bruxellensis using time consuming plate counting. B. bruxellensis is a fastidious slow growing organism, requiring five days of incubation at 30°C for visible growth on agar plates. Thus, a need exists to develop a quicker, feasible method to enumerate this yeast. The aim of this study was therefore to determine the feasibility of using the ‘direct’ and ‘indirect’ impedance methods for the enumeration of B. bruxellensis in beer and to monitor the growth of the yeast during fermentation. The impedance methods were able to decrease the incubation time of beer samples containing Brettanomyces from 120 h down to 2 and 84 h for samples containing 107 and 103 cfu/mL, respectively. The ‘indirect’ method was more successful than the ‘direct’ method, presenting a smaller error and wider detection range. Overall, the ‘indirect’ impedance method is a viable alternative to plate counting for the enumeration of yeasts in the brewing industry because it decreases preparation and incubation times, thereby increasing throughput and decreasing the chance of contamination. en
dc.publisher Wiley - VCH Verlag GmbH & CO. KGaA en
dc.relation.ispartofseries Biotechnology Journal en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.title Impedance technology reduces the enumeration time of Brettanomyces yeast during beer fermentation en
dc.type Journal Article en
dc.identifier.doi 10.1002/biot.201600497 en
pubs.issue 12 en
pubs.begin-page 1667 en
pubs.volume 11 en
dc.rights.holder Copyright: Wiley en
dc.identifier.pmid 27778472 en
pubs.end-page 1672 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 606535 en
pubs.record-created-at-source-date 2017-05-01 en 2016-11-28 en
pubs.dimensions-id 27778472 en

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