Volumetric characterisation of contracting cardiac trabeculae

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dc.contributor.advisor Taberner, A en
dc.contributor.advisor Nielsen, P en
dc.contributor.author Cheuk, Ming en
dc.date.accessioned 2017-05-04T23:09:20Z en
dc.date.issued 2017 en
dc.identifier.uri http://hdl.handle.net/2292/32776 en
dc.description.abstract Experimental studies performed on isolated cardiac muscle are crucial for understanding the mechanisms of heart contraction. Not only do they reveal the sub-cellular processes that occur during a normal contraction, but also the changes that occur with disease. An understanding of these processes is crucial for the prevention and treatment of heart diseases. Experimental studies are usually performed on samples of different shapes and sizes, requiring that volume dependent measurements such as force and heat-rate be normalised to crosssectional area or volume in order to compare data between samples. However, many research groups are limited to estimating the shape and size of trabeculae by assuming an elliptic cylindrical geometry of the muscle. This simplification falls short when the cross sections of the muscle cannot be appropriately modelled by an ellipse. The accuracy also varies considerably depending on how the muscle is mounted. This thesis addresses the problem of normalisation by using optical coherence tomography (OCT) to image the 3D volume of the trabecula during an experiment, and by quantifying the cross-sectional areas and volume of the tissue using an image processing pipeline. An additional challenge arises from the non-uniform strain commonly developed during contraction, which is accompanied by irregular deformation of the volume. To measure this deformation, the OCT was extended to image a contracting trabecula, by synchronising the capture of cross-sectional images with the stimulation of the muscle. This approach allowed the collection of the first volumetric quantification of trabecular surface geometry during contraction. To further analyse the strain field developed during contraction, transmission images of the muscle were captured, and muscle deformation quantified using digital image correlation (DIC). Measurements of volume and strain during contraction were combined with measurements of force, length, heat-rate, and sarcomere length in a single experiment in order to demonstrate the ability to collect and synchronise measurements across all modalities. The instrumentation and analysis methods developed in this thesis promise to provide greater insight into the mechanical and energetic properties and function of living samples of cardiac muscle tissue, in both health and disease. en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.relation.isreferencedby UoA99264954414102091 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/nz/ en
dc.title Volumetric characterisation of contracting cardiac trabeculae en
dc.type Thesis en
thesis.degree.discipline Bioengineering en
thesis.degree.grantor The University of Auckland en
thesis.degree.level Doctoral en
thesis.degree.name PhD en
dc.rights.holder Copyright: The author en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.elements-id 624381 en
pubs.record-created-at-source-date 2017-05-05 en

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