Assessing Bioactive Extracts from Puriri Leaves for their Antioxidant and Antimicrobial Activities for Possible Use as Varroa Mite Control

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dc.contributor.advisor Hemar, Y en Cao, Yifei en 2017-06-13T23:57:47Z en 2017 en
dc.identifier.uri en
dc.description Full text is available to authenticated members of The University of Auckland only. en
dc.description.abstract Over the past decades, honeybees have been on the decline which is brought on by many causes such as pests, microorganisms, etc. A significant pest of the honeybees includes the Varroa mite. This worldwide pest can cause severe damage to honeybees or cause destruction of bee colonies by spreading pathogenic microorganisms and devouring their host’s nutrients. Previous studies have shown that a way to control Varroa is through the use of plants that express bioactive properties. Such botanical species include the Vitex lucens, also is known as Puriri. Puriri is a tree that is an endemic and native species to New Zealand, which is listed in the Rongoa Māori or the Medical Native Plants of New Zealand. It has historically been used to treat pain in different areas of the body, improve blood and nutritional flow throughout, as well as having an antibacterial characteristic. In this study, puriri leaves were extracted using several solvents (water, ethanol and cyclohexane). A novel technique of using ultrasound was also applied for improving the aqueous extraction yield. The extracts of puriri leaves were investigated for their bioactive properties which can be used for Varroa control. The antioxidant activity was determined by three assays including total phenolic contents (TPC) assay, DPPH assay and ABTS assay. All extracts (water extract with the process of maceration, ultrasound and reflux, water extract with maceration alone, ethanol extract and lipid extract) presented antioxidant properties and high TPC value. The antimicrobial activity test was performed using the disc diffusion method and modified dilution method against bacteria such as Pseudomonas fluorescens, Enterococcus faecalis, Bacillus subtilis, Bacillus cereus, Enterobacter aerogenes, S. epidermidis and Escherichia coli. All puriri leaves extracts had a variable degree of antimicrobial activity against bacteria related to honeybee or Varroa. B. cereus also known as a food-borne pathogen, was found to be the most susceptible to all of the puriri leaves extracts. The antioxidant and antimicrobial properties of the puriri leaves extracts determined in this study offer the direction of developing the anti-Varroa products for the honeybee industry in the future. en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof Masters Thesis - University of Auckland en
dc.relation.isreferencedby UoA99264925808002091 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights Restricted Item. Available to authenticated members of The University of Auckland. en
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dc.title Assessing Bioactive Extracts from Puriri Leaves for their Antioxidant and Antimicrobial Activities for Possible Use as Varroa Mite Control en
dc.type Thesis en Food Science en The University of Auckland en Masters en
dc.rights.holder Copyright: The author en
pubs.elements-id 629897 en
pubs.record-created-at-source-date 2017-06-14 en

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