dc.contributor.author |
Fowke, TM |
en |
dc.contributor.author |
Karunasinghe, Rashika |
en |
dc.contributor.author |
Bai, Jizhong |
en |
dc.contributor.author |
Jordan, S |
en |
dc.contributor.author |
Gunn, Alistair |
en |
dc.contributor.author |
Dean, Justin |
en |
dc.date.accessioned |
2017-06-26T03:40:07Z |
en |
dc.date.issued |
2017-03-13 |
en |
dc.identifier.citation |
Scientific Reports 7: 44135 13 Mar 2017 |
en |
dc.identifier.issn |
2045-2322 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/33759 |
en |
dc.description.abstract |
Hyaluronan is a linear glycosaminoglycan that forms the backbone of perineuronal nets around neurons in the cerebral cortex. However, it remains controversial whether neurons are capable of independent hyaluronan synthesis. Herein, we examined the expression of hyaluronan and hyaluronan synthases (HASs) throughout cortical neuron development in vitro. Enriched cultures of cortical neurons were established from E16 rats. Neurons were collected at days in vitro (DIV) 0 (4 h), 1, 3, 7, 14, and 21 for qPCR or immunocytochemistry. In the relative absence of glia, neurons exhibited HAS1-3 mRNA at all time-points. By immunocytochemistry, puncta of HAS2-3 protein and hyaluronan were located on neuronal cell bodies, neurites, and lamellipodia/growth cones from as early as 4 h in culture. As neurons matured, hyaluronan was also detected on dendrites, filopodia, and axons, and around synapses. Percentages of hyaluronan-positive neurons increased with culture time to ∼93% by DIV21, while only half of neurons at DIV21 expressed the perineuronal net marker Wisteria floribunda agglutinin. These data clearly demonstrate that neurons in vitro can independently synthesise hyaluronan throughout all maturational stages, and that hyaluronan production is not limited to neurons expressing perineuronal nets. The specific structural localisation of hyaluronan suggests potential roles in neuronal development and function. |
en |
dc.publisher |
Nature Publishing Group |
en |
dc.relation.ispartofseries |
Scientific Reports |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/2045-2322/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
en |
dc.title |
Hyaluronan synthesis by developing cortical neurons in vitro |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1038/srep44135 |
en |
pubs.volume |
7 |
en |
dc.description.version |
VoR - Version of Record |
en |
dc.rights.holder |
Copyright: The Authors |
en |
dc.identifier.pmid |
28287145 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
618525 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Physiology Division |
en |
dc.identifier.eissn |
2045-2322 |
en |
pubs.record-created-at-source-date |
2017-03-25 |
en |
pubs.dimensions-id |
28287145 |
en |