dc.contributor.author |
Tsai, Jia Yun |
en |
dc.contributor.author |
Hughes, Jacelyn |
en |
dc.contributor.author |
Clow, F |
en |
dc.contributor.author |
Lorenz, Natalie |
en |
dc.contributor.author |
Proft, Thomas |
en |
dc.date.accessioned |
2017-08-11T00:13:20Z |
en |
dc.date.issued |
2017-01 |
en |
dc.identifier.citation |
Molecular Microbiology 103(2):282-298 Jan 2017 |
en |
dc.identifier.issn |
0950-382X |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/35017 |
en |
dc.description.abstract |
Group A Streptococcus (GAS), or Streptococcus pyogenes, is a human pathogen that causes diseases ranging from skin and soft tissue infections to severe invasive diseases, such as toxic shock syndrome. Each GAS strain carries a particular pilus type encoded in the variable fibronectin-binding, collagen-binding, T antigen (FCT) genomic region. Here, we describe the functional analysis of the serotype M2 pilus encoded in the FCT-6 region. We found that, in contrast to other investigated GAS pili, the ancillary pilin 1 lacks adhesive properties. Instead, the backbone pilin is important for host cell adhesion and binds several host factors, including fibronectin and fibrinogen. Using a panel of recombinant pilus proteins, GAS gene deletion mutants and Lactococcus lactis gain-of-function mutants we show that, unlike other GAS pili, the FCT-6 pilus also contributes to immune evasion. This was demonstrated by a delay in blood clotting, increased intracellular survival of the bacteria in macrophages, higher bacterial survival rates in human whole blood and greater virulence in a Galleria mellonella infection model in the presence of fully assembled FCT-6 pili. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.publisher |
Blackwell Publishing Inc. |
en |
dc.relation.ispartofseries |
Molecular Microbiology |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
The Group A Streptococcus serotype M2 pilus plays a role in host cell adhesion and immune evasion |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1111/mmi.13556 |
en |
pubs.issue |
2 |
en |
pubs.begin-page |
282 |
en |
pubs.volume |
103 |
en |
dc.rights.holder |
Copyright: Blackwell Publishing Inc. |
en |
dc.identifier.pmid |
27741558 |
en |
pubs.end-page |
298 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
543704 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1365-2958 |
en |
pubs.record-created-at-source-date |
2017-08-11 |
en |
pubs.dimensions-id |
27741558 |
en |