The Group A Streptococcus serotype M2 pilus plays a role in host cell adhesion and immune evasion

Show simple item record Tsai, Jia Yun en Hughes, Jacelyn en Clow, F en Lorenz, Natalie en Proft, Thomas en 2017-08-11T00:13:20Z en 2017-01 en
dc.identifier.citation Molecular Microbiology 103(2):282-298 Jan 2017 en
dc.identifier.issn 0950-382X en
dc.identifier.uri en
dc.description.abstract Group A Streptococcus (GAS), or Streptococcus pyogenes, is a human pathogen that causes diseases ranging from skin and soft tissue infections to severe invasive diseases, such as toxic shock syndrome. Each GAS strain carries a particular pilus type encoded in the variable fibronectin-binding, collagen-binding, T antigen (FCT) genomic region. Here, we describe the functional analysis of the serotype M2 pilus encoded in the FCT-6 region. We found that, in contrast to other investigated GAS pili, the ancillary pilin 1 lacks adhesive properties. Instead, the backbone pilin is important for host cell adhesion and binds several host factors, including fibronectin and fibrinogen. Using a panel of recombinant pilus proteins, GAS gene deletion mutants and Lactococcus lactis gain-of-function mutants we show that, unlike other GAS pili, the FCT-6 pilus also contributes to immune evasion. This was demonstrated by a delay in blood clotting, increased intracellular survival of the bacteria in macrophages, higher bacterial survival rates in human whole blood and greater virulence in a Galleria mellonella infection model in the presence of fully assembled FCT-6 pili. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.publisher Blackwell Publishing Inc. en
dc.relation.ispartofseries Molecular Microbiology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.title The Group A Streptococcus serotype M2 pilus plays a role in host cell adhesion and immune evasion en
dc.type Journal Article en
dc.identifier.doi 10.1111/mmi.13556 en
pubs.issue 2 en
pubs.begin-page 282 en
pubs.volume 103 en
dc.rights.holder Copyright: Blackwell Publishing Inc. en
dc.identifier.pmid 27741558 en
pubs.end-page 298 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 543704 en Medical and Health Sciences en Medical Sciences en Molecular Medicine en Science en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1365-2958 en
pubs.record-created-at-source-date 2017-08-11 en
pubs.dimensions-id 27741558 en

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