First proteomic profiling of exosomes in rodent intestinal lymph

Show simple item record Hong, Jiwon en Nachkebia, S en Tun, SM en Premkumar, R en Blenkiron, C en Windsor, John en Phillips, Anthony en
dc.coverage.spatial Rotterdam, The Netherlands en 2017-08-17T02:23:14Z en 2016-05-06 en
dc.identifier.citation The 5th International meeting of International Society for Extracellular Vesicles (ISEV). 06 May 2016 en
dc.identifier.uri en
dc.description.abstract Introduction: Exosomes are released by many cell types and can be taken up by other cells. They may play an important role in cell-to-cell communication and disease pathogenesis. Exosomes derived from plasma or urine have been extensively studied, but that of intestinal lymph has not been reported due to the difficulty in obtaining these samples. Intestinal lymph is continuously draining from the intestine and enters the veins just before the heart and lungs. These organs may therefore be directly influenced by intestinal exosomes. Methods: Intestinal lymph was collected from a rodent experimental model of critical illness. Exosomes were isolated from the intestinal lymph using a commercially available exosome isolation kit. Particle size and concentration were determined by Nanosight. Proteomic profiling of lymph exosomes and its changes in critical illness were analysed by LC-MS. Results: The size and concentration of “exosomes” isolated from the intestinal lymph did not change significantly in the critical illness. The exosome preparation contained proteins previously identified in microparticles or exosomes (e.g. inter-alpha trypsin inhibitor), but also the highly abundant plasma proteins (e.g. complement C3, albumin). Common exosomal markers (e.g. TSG101, CD63) were not detected. Instead, substantial amounts of Apo B and A-I were found, indicating presumed co-isolation of chylomirons. Lymph chylomicrons are similar to size of “exosomes”, and produced in high concentration from the intestine. Our study indicates the unexpected difficulty in isolating pure exosomes using a commercial kit in this unique fluid. Conclusion: This present study provides the first attempt at a proteomic profile of an exosome preparation from intestinal lymph. Collectively, multiple proteins were identified, but found to have come from both exosomes and chylomicrons. New purification methods will be needed to study pure isolates of each particle type in this unique fluid. en
dc.relation.ispartof The 5th International meeting of International Society for Extracellular Vesicles (ISEV) en
dc.relation.ispartofseries Journal of Extracellular Vesicles, Vol. 5, Suppl. 1 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.title First proteomic profiling of exosomes in rodent intestinal lymph en
dc.type Conference Poster en
dc.rights.holder Copyright: The authors en en
dc.rights.accessrights en
pubs.elements-id 539786 en Medical and Health Sciences en Medical Sciences en Molecular Medicine en School of Medicine en Surgery Department en Science en Biological Sciences en Science Research en Maurice Wilkins Centre (2010-2014) en
pubs.record-created-at-source-date 2016-08-18 en

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