dc.contributor.author |
Raos, BJ |
en |
dc.contributor.author |
Graham, ES |
en |
dc.contributor.author |
Unsworth, CP |
en |
dc.coverage.spatial |
Orlando, FL, USA |
en |
dc.date.accessioned |
2017-10-03T00:58:26Z |
en |
dc.date.issued |
2016 |
en |
dc.identifier.citation |
2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), proceedings of the conference held 16 - 20 August 2016, Orlando, Florida, pp. 1750-1753 |
en |
dc.identifier.isbn |
978-1-4577-0220-4 |
en |
dc.identifier.issn |
1557-170X |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/35836 |
en |
dc.description.abstract |
We demonstrate, for the first time, UV laser ablative microsurgery as a method for pruning astrocytic processes from live hNT astrocytic networks in vitro. Calcium fluorescence imaging was used to evaluate the cellular response to process ablation. The results showed that ablation of astrocyte processes induced an immediate increase in intracellular calcium level which propagated through the cells cytoplasm as a wave originating from the ablation site. The increased intracellular calcium dissipated from the body of the cell but remained high in the vicinity of the ablation site. Cell viability post ablation was confirmed by observing the integrity of the cell membrane. Ablation of astrocytic processes did not compromise cell viability whereas ablation of the cytoplasm using the same laser energy resulted in cell lysis. |
en |
dc.description.uri |
https://embc.embs.org/2016/ |
en |
dc.publisher |
Institute of Electrical and Electronics Engineers |
en |
dc.relation.ispartof |
38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society |
en |
dc.relation.ispartofseries |
2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.ieee.org/publications_standards/publications/rights/rights_policies.html |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Investigation of the Ca2+ Response of human hNT astrocytes to laser removal of cellular processes |
en |
dc.type |
Conference Item |
en |
dc.identifier.doi |
10.1109/EMBC.2016.7591055 |
en |
pubs.begin-page |
1750 |
en |
dc.rights.holder |
Copyright: Institute of Electrical and Electronics Engineers |
en |
dc.identifier.pmid |
28268665 |
en |
dc.identifier.pmid |
28226849 |
en |
pubs.author-url |
http://ieeexplore.ieee.org/document/7591055/ |
en |
pubs.end-page |
1753 |
en |
pubs.finish-date |
2016-08-20 |
en |
pubs.publication-status |
Published |
en |
pubs.start-date |
2016-08-16 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Proceedings |
en |
pubs.elements-id |
617645 |
en |
pubs.org-id |
Engineering |
en |
pubs.org-id |
Engineering Science |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
Pharmacy |
en |
dc.identifier.eissn |
1558-4615 |
en |
pubs.record-created-at-source-date |
2016-08-02 |
en |
pubs.dimensions-id |
28268665 |
en |