Age and sex differences in human skeletal muscle fibrosis markers and transforming growth factor-β signaling

Show simple item record Parker, L en Caldow, MK en Watts, R en Levinger, P en Cameron-Smith, David en Levinger, I en 2017-10-16T21:44:41Z en 2017-07 en
dc.identifier.citation European Journal of Applied Physiology 117(7):1463-1472 Jul 2017 en
dc.identifier.issn 1439-6319 en
dc.identifier.uri en
dc.description.abstract The aim of the study was to determine whether higher fibrosis markers in skeletal muscle of older adults are accompanied by increased expression of components of the canonical TGF-β signal transduction pathway. Fourteen healthy young (21-35 years; 9 males and 5 females) and seventeen older (55-75 years; 9 males and 8 females) participants underwent vastus lateralis biopsies to determine intramuscular mRNA and protein expression of fibrogenic markers and TGF-β signaling molecules related to TGF-β1 and myostatin.Expression of mRNA encoding the pro-fibrotic factors; axin 2, collagen III, β-catenin and fibronectin, were all significantly higher (all p < 0.05) in the older participants (350, 170, 298, and 641%, respectively). Furthermore, axin 2 and β-catenin mRNA were significantly higher in older females than older males (p < 0.05). Gene expression of ActRIIB, myostatin, and TGF-β1 were higher in older adults compared to younger adults (all p < 0.05). There was, however, no difference in the total protein content of myostatin, myoD or myogenin (all p > 0.05), whereas Smad3 protein phosphorylation was 48% lower (p < 0.05) in muscle from older adults.Increased abundance of mRNA of fibrotic markers was observed in muscle from older adults and was partly accompanied by altered abundance of pro-fibrotic ligands in a sex specific manner. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.publisher Springer Verlag en
dc.relation.ispartofseries European Journal of Applied Physiology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.title Age and sex differences in human skeletal muscle fibrosis markers and transforming growth factor-β signaling en
dc.type Journal Article en
dc.identifier.doi 10.1007/s00421-017-3639-4 en
pubs.issue 7 en
pubs.begin-page 1463 en
pubs.volume 117 en
dc.rights.holder Copyright: Springer Verlag en
dc.identifier.pmid 28493029 en
pubs.end-page 1472 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 625612 en
dc.identifier.eissn 1439-6327 en
pubs.record-created-at-source-date 2017-10-17 en
pubs.dimensions-id 28493029 en

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