dc.contributor.author |
Parker, L |
en |
dc.contributor.author |
Caldow, MK |
en |
dc.contributor.author |
Watts, R |
en |
dc.contributor.author |
Levinger, P |
en |
dc.contributor.author |
Cameron-Smith, David |
en |
dc.contributor.author |
Levinger, I |
en |
dc.date.accessioned |
2017-10-16T21:44:41Z |
en |
dc.date.issued |
2017-07 |
en |
dc.identifier.citation |
European Journal of Applied Physiology 117(7):1463-1472 Jul 2017 |
en |
dc.identifier.issn |
1439-6319 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/36104 |
en |
dc.description.abstract |
The aim of the study was to determine whether higher fibrosis markers in skeletal muscle of older adults are accompanied by increased expression of components of the canonical TGF-β signal transduction pathway. Fourteen healthy young (21-35 years; 9 males and 5 females) and seventeen older (55-75 years; 9 males and 8 females) participants underwent vastus lateralis biopsies to determine intramuscular mRNA and protein expression of fibrogenic markers and TGF-β signaling molecules related to TGF-β1 and myostatin.Expression of mRNA encoding the pro-fibrotic factors; axin 2, collagen III, β-catenin and fibronectin, were all significantly higher (all p < 0.05) in the older participants (350, 170, 298, and 641%, respectively). Furthermore, axin 2 and β-catenin mRNA were significantly higher in older females than older males (p < 0.05). Gene expression of ActRIIB, myostatin, and TGF-β1 were higher in older adults compared to younger adults (all p < 0.05). There was, however, no difference in the total protein content of myostatin, myoD or myogenin (all p > 0.05), whereas Smad3 protein phosphorylation was 48% lower (p < 0.05) in muscle from older adults.Increased abundance of mRNA of fibrotic markers was observed in muscle from older adults and was partly accompanied by altered abundance of pro-fibrotic ligands in a sex specific manner. |
en |
dc.format.medium |
Print-Electronic |
en |
dc.language |
eng |
en |
dc.publisher |
Springer Verlag |
en |
dc.relation.ispartofseries |
European Journal of Applied Physiology |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Age and sex differences in human skeletal muscle fibrosis markers and transforming growth factor-β signaling |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1007/s00421-017-3639-4 |
en |
pubs.issue |
7 |
en |
pubs.begin-page |
1463 |
en |
pubs.volume |
117 |
en |
dc.rights.holder |
Copyright: Springer Verlag |
en |
dc.identifier.pmid |
28493029 |
en |
pubs.end-page |
1472 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
625612 |
en |
dc.identifier.eissn |
1439-6327 |
en |
pubs.record-created-at-source-date |
2017-10-17 |
en |
pubs.dimensions-id |
28493029 |
en |