Staphylococcus and the Microbiome in Chronic Rhinosinusitis

Show simple item record

dc.contributor.advisor Taylor, M en
dc.contributor.advisor Biswas, K en
dc.contributor.advisor Hoggard, M en
dc.contributor.advisor Wagner, B en Baker, Jesse en 2018-01-31T23:33:45Z en 2018 en
dc.identifier.uri en
dc.description Full text is available to authenticated members of The University of Auckland only. en
dc.description.abstract Chronic rhinosinusitis (CRS) is a clinical condition resulting in prolonged inflammation of the sinonasal mucosa. Typical symptoms include nasal obstruction and discharge, facial pain, or olfactory dysfunction. These symptoms are sometimes alleviated by treatment with antibiotics, nasal steroids or saline nasal irrigation; however, in severe cases endoscopic surgery is required. CRS is common within the Western world, where it affects ~5% of the population. Despite its prevalence, the etiology and pathogenesis of CRS remains unclear, partially due to its heterogeneous nature of disease presentation. As a result, numerous host and exogenouslybased mechanisms have been hypothesised. The sinonasal microbiota has been implicated in CRS pathogenesis, particularly related to the presence of absence of Staphylococcus aureus. Staphylococcus epidermidis is also prevalent within the sinonasal microbiota and has been shown to potentially inhibit S. aureus biofilm formation. The first part of this study used Illumina sequencing targeted at the bacterial 16S rRNA gene of healthy and CRS-affected individuals to investigate the structure and diversity of the sinonasal microbiota. Quantitative PCR using species-specific and bacterial 16S rRNA primers was used for absolute quantification of S. aureus, S. epidermidis, and bacterial load. Results suggested that sinus microbiota composition was not significantly different between healthy and CRS-affected individuals. S. aureus and S. epidermidis abundance was not correlated with CRS, yet bacterial load was significantly higher in CRS-affected patients. The second part of this study aimed to compare the effects of intranasal Augmentin treatment and/or bacterial inoculation with Pseudomonas aeruginosa and Corynebacterium tuberculostearicum, two species whose presence is positively correlated with CRS, on the mouse sinus, lung, and gut microbiota composition as part of broader research efforts to develop a new mouse model of CRS. All treatments did not influence gut microbiota composition whilst sinus and lung communities could not be described due to the low proportion of reads taxonomically identified past Domain level. This study contributes to current knowledge surrounding the composition of human microbial communities in CRS and is hoped to aid in efforts to improve the quality of life for individuals suffering from CRS. en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof Masters Thesis - University of Auckland en
dc.relation.isreferencedby UoA99265058010602091 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights Restricted Item. Available to authenticated members of The University of Auckland. en
dc.rights.uri en
dc.rights.uri en
dc.title Staphylococcus and the Microbiome in Chronic Rhinosinusitis en
dc.type Thesis en Biological Sciences en The University of Auckland en Masters en
dc.rights.holder Copyright: The author en
pubs.elements-id 722772 en
pubs.record-created-at-source-date 2018-02-01 en
dc.identifier.wikidata Q112935566

Files in this item

Find Full text

This item appears in the following Collection(s)

Show simple item record


Search ResearchSpace