New Zealand Fungi: The hunt for novel antibiotics

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dc.contributor.advisor Wiles, S en
dc.contributor.author Tan, Tze How en
dc.date.accessioned 2018-08-01T02:24:12Z en
dc.date.issued 2018 en
dc.identifier.uri http://hdl.handle.net/2292/37580 en
dc.description Full text is available to authenticated members of The University of Auckland only. en
dc.description.abstract Antibiotic resistance has become a global public health threat and current action plans in place worldwide are mainly focused on preventing the development of resistance to current antibiotics. The discovery of novel antibiotic classes is crucial to solve this crisis. In this project, I have explored New Zealand fungi as a potential source of new antibiotic. New Zealand has a very isolated geography in the world and is home to many unique species, including flightless birds like the kiwi. I hypothesise that New Zealand may also be home to fungal species producing unknown secondary metabolites with antibiotic properties. As standard pure culture techniques may not trigger the production of such secondary metabolites, I have co-cultured fungal isolates with three bacterial species (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) to encourage competition during growth to increase the probability of locating an antimicrobial compound. I have also used bioluminescently-tagged bacteria, so that I can use bacterial light production as a surrogate for bacterial viability. Reduction in bioluminescence indicates inhibitory activity from the fungi as dead or metabolically inactive bacteria no longer produce light. I complemented this assay by also testing fungal isolates for the ability to produce zones of inhibition when grown with the bacteria. Of the 143 fungal isolates I have screened in triplicates, I have identified 37 with activity against S. aureus, 5 with activity against E. coli, and 6 with activity against P. aeruginosa. I prepared extracts using reverse chromatography from 12 of the fungal isolates that affected light production and showed excellent bacterial inhibition in the zone of inhibition tests. Overall, I did not discover any new types of antibiotics over the course of my project. However, my results show that our screening method is effective and can facilitate the future discovery of novel antibiotics from New Zealand fungi. en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof Masters Thesis - University of Auckland en
dc.relation.isreferencedby UoA99265073209502091 en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights Restricted Item. Available to authenticated members of The University of Auckland. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/nz/ en
dc.title New Zealand Fungi: The hunt for novel antibiotics en
dc.type Thesis en
thesis.degree.discipline Biomedical Science en
thesis.degree.grantor The University of Auckland en
thesis.degree.level Masters en
dc.rights.holder Copyright: The author en
pubs.elements-id 750632 en
pubs.record-created-at-source-date 2018-08-01 en


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http://creativecommons.org/licenses/by-nc-sa/3.0/nz/ Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/3.0/nz/

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