dc.contributor.author |
Sander, Veronika |
en |
dc.contributor.author |
Patke, Shantanu |
en |
dc.contributor.author |
Lee, Jung Y |
en |
dc.contributor.author |
Chang, Young-Tae |
en |
dc.contributor.author |
Davidson, Alan |
en |
dc.date.accessioned |
2018-10-10T20:46:08Z |
en |
dc.date.issued |
2017-03-13 |
en |
dc.identifier.issn |
1420-3049 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/40510 |
en |
dc.description.abstract |
We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals. |
en |
dc.format.medium |
Electronic |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Molecules (Basel, Switzerland) |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1420-3049/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Intestines |
en |
dc.subject |
Animals |
en |
dc.subject |
Zebrafish |
en |
dc.subject |
Boron Compounds |
en |
dc.subject |
Tretinoin |
en |
dc.subject |
Microscopy, Fluorescence |
en |
dc.subject |
Signal Transduction |
en |
dc.subject |
Larva |
en |
dc.title |
The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.3390/molecules22030454 |
en |
pubs.issue |
3 |
en |
pubs.volume |
22 |
en |
dc.rights.holder |
Copyright: The authors |
en |
dc.identifier.pmid |
28335401 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
research-article |
en |
pubs.subtype |
Journal Article |
en |
pubs.elements-id |
618036 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
dc.identifier.eissn |
1420-3049 |
en |
pubs.record-created-at-source-date |
2017-03-25 |
en |
pubs.dimensions-id |
28335401 |
en |