Crystal structure of a bicupin protein HutD involved in histidine utilization in Pseudomonas.

Show simple item record Gerth, ML en Liu, Y en Jiao, W en Zhang, X-X en Baker, Edward en Lott, Jeremy en Rainey, PB en Johnston, Jodie en 2018-10-10T23:28:04Z en 2017-08 en
dc.identifier.issn 0887-3585 en
dc.identifier.uri en
dc.description.abstract Cupins form one of the most functionally diverse superfamilies of proteins, with members performing a wide range of catalytic, non-catalytic, and regulatory functions. HutD is a predicted bicupin protein that is involved in histidine utilization (Hut) in Pseudomonas species. Previous genetic analyses have suggested that it limits the upper level of Hut pathway expression, but its mechanism of action is unknown. Here, we have determined the structure of PfluHutD at 1.74 Å resolution in several crystallization conditions, and identified N-formyl-l-glutamate (FG, a Hut pathway intermediate) as a potential ligand in vivo. Proteins 2017; 85:1580-1588. © 2017 Wiley Periodicals, Inc. en
dc.format.medium Print-Electronic en
dc.language eng en
dc.relation.ispartofseries Proteins en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.subject Pseudomonas fluorescens en
dc.subject Escherichia coli en
dc.subject Glutamates en
dc.subject Histidine en
dc.subject Bacterial Proteins en
dc.subject Recombinant Proteins en
dc.subject Crystallography, X-Ray en
dc.subject Cloning, Molecular en
dc.subject Gene Expression en
dc.subject Binding Sites en
dc.subject Amino Acid Motifs en
dc.subject Protein Binding en
dc.subject Biological Transport en
dc.subject Models, Molecular en
dc.subject Protein Interaction Domains and Motifs en
dc.subject Protein Conformation, alpha-Helical en
dc.subject Protein Conformation, beta-Strand en
dc.title Crystal structure of a bicupin protein HutD involved in histidine utilization in Pseudomonas. en
dc.type Journal Article en
dc.identifier.doi 10.1002/prot.25303 en
pubs.issue 8 en
pubs.begin-page 1580 en
pubs.volume 85 en
dc.rights.holder Copyright: The author en
dc.identifier.pmid 28383128 en
pubs.end-page 1588 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Journal Article en
pubs.elements-id 622718 en Science en Biological Sciences en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1097-0134 en
pubs.record-created-at-source-date 2017-04-06 en
pubs.dimensions-id 28383128 en

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