In vitro and ex vivo characterisation of an in situ gelling formulation for sustained lidocaine release with potential use following knee arthroplasty.

Show simple item record Sharma, Manisha en Chandramouli, Kaushik en Curley, Louise en Pontre, Beau en Reilly, Keryn en Munro, Jacob en Hill, Andrew en Young, Simon en Svirskis, Darren en 2018-10-14T22:12:51Z en 2018-06 en
dc.identifier.issn 2190-393X en
dc.identifier.uri en
dc.description.abstract Sustained lidocaine release via a thermoresponsive poloxamer-based in situ gelling system has the potential to alleviate pain following knee arthroplasty. A previously developed formulation showed a promising drug release profile in synthetic phosphate-buffered saline (PBS). To support the translation of this formulation, ex vivo characterisation was warranted. This study therefore aimed (1) to modify the previously developed formulation to reduce the burst release, (2) to compare the release behaviour into ex vivo human intra-articular fluid (IAF) and PBS and (3) to determine the formulation spread in an ex vivo human knee using magnetic resonance imaging (MRI). All formulations provided sustained release out to 72 h; polyvinyl pyrrolidone was the most effective additive yielding a small yet significant decrease (p < 0.05) in the burst release. Release of lidocaine from the formulation occurred significantly faster into IAF compared to PBS (1.4 times greater release in the first 24 h), correlating with faster rates of gel erosion in IAF. Injection was easily achieved through a 21-gauge (G) needle into the synovial space of a human cadaveric knee, and MRI scans revealed effective spreading of the formulation throughout the joint cavity. The pattern of spread is promising for the drug to reach the widespread nerve endings in the joint capsule; the effect of this spread on release in an in vivo setting will be the subject of future studies. The demonstrated properties indicate that the in situ gelling formulation has the potential to be used clinically to treat post-operative pain following knee arthroplasty. en
dc.format.medium Print en
dc.language eng en
dc.relation.ispartofseries Drug delivery and translational research en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.subject Knee Joint en
dc.subject Humans en
dc.subject Poloxamer en
dc.subject Lidocaine en
dc.subject Povidone en
dc.subject Anesthetics, Local en
dc.subject Gels en
dc.subject Delayed-Action Preparations en
dc.subject Magnetic Resonance Imaging en
dc.subject Arthroplasty, Replacement, Knee en
dc.subject Temperature en
dc.subject Drug Liberation en
dc.title In vitro and ex vivo characterisation of an in situ gelling formulation for sustained lidocaine release with potential use following knee arthroplasty. en
dc.type Journal Article en
dc.identifier.doi 10.1007/s13346-018-0492-x en
pubs.issue 3 en
pubs.begin-page 820 en
pubs.volume 8 en
dc.rights.holder Copyright: The author en
dc.identifier.pmid 29411295 en
pubs.end-page 829 en
pubs.publication-status Published en
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't en
pubs.subtype Journal Article en
pubs.elements-id 725218 en Medical and Health Sciences en Medical Sciences en Anatomy and Medical Imaging en Pharmacy en School of Medicine en South Auckland clinical school en Surgery Department en
dc.identifier.eissn 2190-3948 en
pubs.record-created-at-source-date 2018-02-08 en
pubs.dimensions-id 29411295 en

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